三七总皂苷干预去势骨质疏松性骨折模型大鼠的作用机制.

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    • Alternate Title:
      Mechanism underlying the interventional effect of Panax Notoginsenosides on ovariectomized osteoporotic fracture rats.
    • Abstract:
      BACKGROUND: Osteoporotic fractures are the most common serious complications caused by osteoporosis, and their repair is more difficult, which seriously threatens the health and quality of life of patients. OBJECTIVE: To investigate the interventional effect of Panax Notoginsenosides in ovariectomized osteoporotic fracture rats and the relevant mechanism. METHODS: Fifty healthy female rats were selected. Ten of them were normal group, and the other 40 rats were used to make ovariectomized osteoporotic fracture models. Model rats were divided into model group, low, medium and high dose group. Normal group and model group rats were intragastrically administered normal saline, rats in the low, medium and high dose group were given intragastric administration of 10, 20 and 40 mg/kg Panax Notoginsenosides solution. The study protocol was approved by the Animal Ethic Committee of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. (2019)58 on September 23, 2019. RESULTS AND CONCLUSION: The bone mineral density and bone cell index of the high dose group were higher than those of low and middle dose groups (P < 0.05). The bone volume fraction, the number of callus trabeculae and the thickness of trabeculae in the high dose group were all higher than those in the model group, the low dose group and the middle dose group, and the resolution of trabeculae was lower than that in these three groups (P < 0.05). The levels of glutathione peroxidase, bone morphogenetic protein 2, and vascular endothelial growth factor in the high dose group were lower than those in the normal group, but higher than those in the model group, low and medium dose groups. The levels of lipid peroxide, malondialdehyde, osteocalcin, type I procollagen carboxy terminal propeptide, bone-specific alkaline phosphatase in the high dose group were higher than those in the normal group, but lower than those in the model group, low and medium dose group (P < 0.05). The expression of PI3K, Akt and mTOR in the low, middle and high dose groups was lower than that in the normal group and higher than that in the model group; and the expression of PI3K, Akt and mTOR in the high dose group was higher than that in the low and middle dose groups (P < 0.05). Therefore, treatment with Panax Notoginsenosides can increase the bone miner density and bone cell index, promote the growth of bone trabecula at the callus, alleviate oxidative stress injury, regulate the PI3K / Akt / mTOR signal pathway, and accelerate the formation of new blood vessels in the callus and fracture healing in ovariectomized osteoporotic fracture rats. [ABSTRACT FROM AUTHOR]
    • Abstract:
      背景:骨质疏松性骨折是最常见的由骨质疏松引起的严重并发症,其修复较为困难,严重威胁患者身体健康、生活质量。 目的:使用三七总皂苷对去势骨质疏松性骨折大鼠进行干预,研究相关作用机制。 方法:选取 50 只健康雌性大鼠,10 只为正常组,其余 40 只建立去势骨质疏松性骨折模型,分为模型组、三七总皂苷低、中、高剂量组, 正常组和模型组大鼠灌胃生理盐水,三七总皂苷低、中、高剂量组大鼠分别灌胃 10,20,40 mg/kg 三七总皂苷溶液。实验于 2019-09-23 经 贵州中医药大学第一附属医院动物实验伦理委员会批准,批准号为 (2019) 伦审第 (58) 号。 结果与结论:三七总皂苷高剂量组大鼠骨密度、骨细胞指数均高于低、中剂量组 (P < 0.05)。高剂量组大鼠骨体积分数、骨痂骨小梁数 量、骨小梁厚度均高于模型组、三七总皂苷低、中剂量组,骨小梁分离度低于模型组、三七总皂苷低、中剂量组 (P < 0.05)。三七总皂苷 高剂量组大鼠谷胱甘肽过氧化物酶、骨形成发生蛋白2、血管内皮生长因子水平低于正常组,高于模型组、三七总皂苷低、中剂量组, 过氧化脂、丙二醛、骨钙素、Ⅰ型前胶原羧基端前肽、骨特异性碱性磷酸酶水平高于正常组,低于模型组以及三七总皂苷低、中剂量组 (P < 0.05)。三七总皂苷低、中、高剂量组大鼠 PI3K、Akt、mTOR表达均低于正常组、高于模型组,且三七总皂苷高剂量组大鼠 PI3K、Akt、 mTOR 表达均高于三七总皂苷低、中剂量组 (P < 0.05)。提示使用三七总皂苷干预后,去势骨质疏松性骨折大鼠骨密度、骨细胞指数上升, 促进骨痂处骨小梁生长,减轻大鼠氧化应激损伤,调控 PI3K/Akt/mTOR 信号通路,促进骨痂新生血管形成及骨折愈合。 [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo zu zhi gong cheng yan jiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)