槲皮素对胫骨平台骨折新西兰兔模型骨形态生成蛋白/Runt相关转录因子2信号通路的影响.

Effects of Quercetin on Bone Morphogenetic Protein / Runt-Related Transcription Factor 2 Signaling Pathway in New Zealand Rabbit Model with Tibial Plateau Fracture.

OBJECTIVE: To study the effects of quercetin on bone morphogenetic protein ( BMP ) / runt-related transcription factor 2(Runx2) signaling pathway in New Zealand rabbit model with tibial plateau fracture, to probe into the mechanism of quercetin and its effects in promoting fracture healing. METHODSS: Totally 40 healthy New Zealand rabbits were selected, 10 rabbits as the normal group and the remaining 30 rabbits were used to establish tibial plateau fracture model, which were randomly divided into the model group, low-dose group and high-dose group. The low-dose group and high-dose group were respectively given 100 mg / kg and 200 mg / kg of quercetin for gavage once a day; the normal group and model group were given an equal volume of 0. 9% NaCl solution once a day; each group was continuously intervened for 7 days. The serum osteocalcin concentrations were measured, the pathomorphism of bone callus tissue were observed, the expression of BMP / Runx2 signaling pathway factors and changes of inflammatory factors [tumor necrosis factor α(TNF-α), interleukin(IL) 1β and IL-10] were detected. RESULTS: Compared with the normal group, the serum osteocalcin levels were decreased, the levels of TNF-α, IL-1β and IL-10 increased in the model group, low-dose group and high-dose group; compared with the model group and low-dose group, the level of osteocalcin was higher, the levels of TNF-α, IL-1β and IL-10 were lower in the high-dose group, with statistically significant differences(P<0. 05). Compared with the normal group, the expression of BMP and Runx2 was higher in the model group, low-dose group and high-dose group; compared with the model group and low-dose group, the expression of BMP and Runx2 was higher in the high-dose group, with statistically significant differences(P<0. 05). CONCLUSIONS: The intervention of qercetin in New Zealand rabbits with tibial plateau fracture can effectively improve the pathological damage of bone callus, inhibit the activity of inflammatory mediators, which is contribute to fracture healing, and the mechanism may be related to the activation of BMP / Runx2 signaling pathway, confirming that quercetin can effectively promote fracture healing with significant effect. [ABSTRACT FROM AUTHOR]

目的：研究槲皮素对胫骨平台骨折新西兰兔模型骨形态生成蛋白（BMP）/Runt相关转录因子2（Runx2）信号通路的影响，探讨槲皮素的作用机制及促进骨折愈合的效果。方法：选取40只健康新西兰大白兔，10只作为正常组，其余30只建立胫骨平台骨折模型，并随机分为模型组、低剂量组和高剂量组。低剂量组、高剂量组新西兰兔分别给予槲皮素100、200 mg/kg灌胃，1日1次；正常组、模型组新西兰兔给予等体积0.9%氯化钠溶液干预，1日1次；各组均连续干预7 d。测量新西兰兔血清骨钙素浓度，观察骨痂组织病理形态，检测BMP/Runx2信号通路因子表达及炎症因子[肿瘤坏死因子α（TNF-α）、白细胞介素（IL）1β和IL-10]变化。结果：与正常组相比，模型组、低剂量组和高剂量组新西兰兔血清骨钙素水平降低，TNF-α、IL-1β和IL-10水平升高；与模型组、低剂量组相比，高剂量组新西兰兔骨钙素水平较高，TNF-α、IL-1β和IL-10水平较低，上述差异均有统计学意义（P<0.05）。与正常组相比，模型组、低剂量组和高剂量组新西兰兔BMP、Runx2表达升高；与模型组、低剂量组相比，高剂量组新西兰兔BMP、Runx2表达升高，上述差异均有统计学意义（P<0.05）。结论：对胫骨平台骨折新西兰兔给予槲皮素干预，可有效改善骨痂病理损伤，抑制炎症介质活性，有助于骨折愈合，其机制可能与激活BMP/Runx2信号通路相关，证实槲皮素可有效促进骨折愈合，效果显著。 [ABSTRACT FROM AUTHOR]

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