Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain.

Item request has been placed! ×
Item request cannot be made. ×
  Processing Request
  • Additional Information
    • Subject Terms:
    • Author-Supplied Keywords:
      antioxidant
      normal tissue complication probability
      pre-conditioning
      radioprotection
    • Abstract:
      Purpose: We examined the effects of manganese (III) meso-tetrakis (diethyl-2-5-imidazole) porphyrin, a metalloporphyrin antioxidant (MPA), on neural tissue radiation toxicity in vivo and on tumour cell radiosensitivity in vitro. Materials and methods: MPA was administered directly into the right lateral ventricle of young adult, male Sprague-Dawley rats (0 or 3.4 μg) 3 h before treatment with a single fraction, 100 Gy radiation dose delivered to the left brain hemisphere. The effects of treatment on radiation responses were assessed at different time points following irradiation. Results: MPA treatment prior to brain irradiation protected against acute radiation-induced apoptosis and ameliorated delayed damage to the blood-brain barrier and radiation necrosis, but without producing a discernible increase in tissue superoxide disumtase (SOD) activity. In vitro, MPA pretreatment protected against radiation-induced apoptosis in primary neuronal cultures and increased clonogenic survival of irradiated rat glioma C6 cells, but had no discernible effect on radiation-induced DNA double-strand breaks. MPA, a low molecular weight SOD mimic, significantly increased mitochondrial SOD activity in C6 cells, but not total cellular SOD activity. MPA up-regulated C6 expression of heme-oxygenase 1 (HO-1), an endogenous radioprotectant, but had no effect on HO-1 levels in human astrocytoma U-251 cells, human prostatic carcinoma LNCaP cells, or primary rat brain microvascular endothelial cells in vitro, nor on brain tissue HO-1 expression levels in vivo. Conclusions: Metalloporphyrin antioxidants merit further exploration as adjunctive radioprotectants for cranial radiotherapy/radiosurgery applications, although the potential for tumour protection must be carefully considered. [ABSTRACT FROM AUTHOR]
    • :
      Copyright of International Journal of Radiation Biology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Full Text Word Count:
      12034
    • ISSN:
      0955-3002
    • DOI:
      10.3109/09553000903419965
    • Accession Number:
      48027041
  • Citations
    • ABNT:
      PEARLSTEIN, R. D. et al. Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain. International Journal of Radiation Biology, [s. l.], v. 86, n. 2, p. 145–163, 2010. Disponível em: . Acesso em: 23 ago. 2019.
    • AMA:
      Pearlstein RD, Higuchi Y, Moldovan M, et al. Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain. International Journal of Radiation Biology. 2010;86(2):145-163. doi:10.3109/09553000903419965.
    • APA:
      Pearlstein, R. D., Higuchi, Y., Moldovan, M., Johnson, K., Fukuda, S., Gridley, D. S., … Slater, J. M. (2010). Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain. International Journal of Radiation Biology, 86(2), 145–163. https://doi.org/10.3109/09553000903419965
    • Chicago/Turabian: Author-Date:
      Pearlstein, Robert D., Yoshinori Higuchi, Maria Moldovan, Kwame Johnson, Shiro Fukuda, Daila S. Gridley, James D. Crapo, David S. Warner, and James M. Slater. 2010. “Metalloporphyrin Antioxidants Ameliorate Normal Tissue Radiation Damage in Rat Brain.” International Journal of Radiation Biology 86 (2): 145–63. doi:10.3109/09553000903419965.
    • Harvard:
      Pearlstein, R. D. et al. (2010) ‘Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain’, International Journal of Radiation Biology, 86(2), pp. 145–163. doi: 10.3109/09553000903419965.
    • Harvard: Australian:
      Pearlstein, RD, Higuchi, Y, Moldovan, M, Johnson, K, Fukuda, S, Gridley, DS, Crapo, JD, Warner, DS & Slater, JM 2010, ‘Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain’, International Journal of Radiation Biology, vol. 86, no. 2, pp. 145–163, viewed 23 August 2019, .
    • MLA:
      Pearlstein, Robert D., et al. “Metalloporphyrin Antioxidants Ameliorate Normal Tissue Radiation Damage in Rat Brain.” International Journal of Radiation Biology, vol. 86, no. 2, Feb. 2010, pp. 145–163. EBSCOhost, doi:10.3109/09553000903419965.
    • Chicago/Turabian: Humanities:
      Pearlstein, Robert D., Yoshinori Higuchi, Maria Moldovan, Kwame Johnson, Shiro Fukuda, Daila S. Gridley, James D. Crapo, David S. Warner, and James M. Slater. “Metalloporphyrin Antioxidants Ameliorate Normal Tissue Radiation Damage in Rat Brain.” International Journal of Radiation Biology 86, no. 2 (February 2010): 145–63. doi:10.3109/09553000903419965.
    • Vancouver/ICMJE:
      Pearlstein RD, Higuchi Y, Moldovan M, Johnson K, Fukuda S, Gridley DS, et al. Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain. International Journal of Radiation Biology [Internet]. 2010 Feb [cited 2019 Aug 23];86(2):145–63. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=c9h&AN=48027041