Effect of DNA complexation and freeze-drying on the physicochemical characteristics of cationic liposomes.

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  • Author(s): Cortesi R;Cortesi R; Esposito E; Nastruzzi C
  • Source:
    Antisense & nucleic acid drug development [Antisense Nucleic Acid Drug Dev] 2000 Jun; Vol. 10 (3), pp. 205-15.
  • Publication Type:
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9606142 Publication Model: Print Cited Medium: Print ISSN: 1087-2906 (Print) Linking ISSN: 10872906 NLM ISO Abbreviation: Antisense Nucleic Acid Drug Dev Subsets: MEDLINE
    • Publication Information:
      Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1996-c2003.
    • Subject Terms:
      DNA/*chemistry ; Liposomes/*chemistry; Binding Sites ; Cations ; Chemical Phenomena ; Chemistry, Physical ; Cryoprotective Agents/pharmacology ; DNA/metabolism ; DNA/ultrastructure ; Dextrans/pharmacology ; Electric Conductivity ; Freeze Drying ; Humans ; Liposomes/metabolism ; Microscopy, Electron, Scanning ; Phosphatidylcholines/chemistry ; Sorbitol/pharmacology ; Sucrose/pharmacology ; Surface Properties ; Time Factors
    • Abstract:
      We describe the use of saccharides, such as sorbitol, mannitol, sucrose, maltodextrin, and dextran, as cyoprotectants for freeze-drying cationic liposomes. Saccharides can protect liposomes either by interacting with phospholipid headgroups or by forming an amorphous glass surrounding the vesicles, thus preventing aggregation, mechanical rupture of membrane, fusion of liposomes, and drug leakage. We have particularly considered liposome characteristics, such as size, zeta potential, and ability in complexing DNA, before and after freeze-drying. Our study indicates that cationic liposomes are able to maintain liposome characteristics after lyophilization and rehydration and maintain the ability to complex DNA even if the strength of the interaction forces was of lower intensity with respect to liposomes before lyophilization.
    • Accession Number:
      0 (Cations)
      0 (Cryoprotective Agents)
      0 (Dextrans)
      0 (Liposomes)
      0 (Phosphatidylcholines)
      506T60A25R (Sorbitol)
      57-50-1 (Sucrose)
      9007-49-2 (DNA)
    • Publication Date:
      Date Created: 20000725 Date Completed: 20001109 Latest Revision: 20161124
    • Publication Date:
      20240104
    • Accession Number:
      10.1089/oli.1.2000.10.205
    • Accession Number:
      10905557