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Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.
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- Author(s): Ho Y;Ho Y; Gruhler A; Heilbut A; Bader GD; Moore L; Adams SL; Millar A; Taylor P; Bennett K; Boutilier K; Yang L; Wolting C; Donaldson I; Schandorff S; Shewnarane J; Vo M; Taggart J; Goudreault M; Muskat B; Alfarano C; Dewar D; Lin Z; Michalickova K; Willems AR; Sassi H; Nielsen PA; Rasmussen KJ; Andersen JR; Johansen LE; Hansen LH; Jespersen H; Podtelejnikov A; Nielsen E; Crawford J; Poulsen V; Sørensen BD; Matthiesen J; Hendrickson RC; Gleeson F; Pawson T; Moran MF; Durocher D; Mann M; Hogue CW; Figeys D; Tyers M
- Source:
Nature [Nature] 2002 Jan 10; Vol. 415 (6868), pp. 180-3.- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print Cited Medium: Print ISSN: 0028-0836 (Print) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
- Publication Information: Publication: Basingstoke : Nature Publishing Group
Original Publication: London, Macmillan Journals ltd. - Subject Terms: Cell Cycle Proteins*; Saccharomyces cerevisiae/*chemistry ; Saccharomyces cerevisiae Proteins/*isolation & purification; Amino Acid Sequence ; Cloning, Molecular ; DNA Damage ; DNA Repair ; DNA, Fungal ; Humans ; Macromolecular Substances ; Mass Spectrometry ; Molecular Sequence Data ; Phosphoric Monoester Hydrolases/metabolism ; Protein Binding ; Protein Kinases/chemistry ; Protein Kinases/metabolism ; Protein Serine-Threonine Kinases ; Proteome ; Saccharomyces cerevisiae Proteins/chemistry ; Sequence Alignment ; Signal Transduction
- Abstract: The recent abundance of genome sequence data has brought an urgent need for systematic proteomics to decipher the encoded protein networks that dictate cellular function. To date, generation of large-scale protein-protein interaction maps has relied on the yeast two-hybrid system, which detects binary interactions through activation of reporter gene expression. With the advent of ultrasensitive mass spectrometric protein identification methods, it is feasible to identify directly protein complexes on a proteome-wide scale. Here we report, using the budding yeast Saccharomyces cerevisiae as a test case, an example of this approach, which we term high-throughput mass spectrometric protein complex identification (HMS-PCI). Beginning with 10% of predicted yeast proteins as baits, we detected 3,617 associated proteins covering 25% of the yeast proteome. Numerous protein complexes were identified, including many new interactions in various signalling pathways and in the DNA damage response. Comparison of the HMS-PCI data set with interactions reported in the literature revealed an average threefold higher success rate in detection of known complexes compared with large-scale two-hybrid studies. Given the high degree of connectivity observed in this study, even partial HMS-PCI coverage of complex proteomes, including that of humans, should allow comprehensive identification of cellular networks.
- Comments: Comment in: Nature. 2002 Jan 10;415(6868):123-4. (PMID: 11805813)
- Accession Number: 0 (Cell Cycle Proteins)
0 (DNA, Fungal)
0 (Macromolecular Substances)
0 (Proteome)
0 (Saccharomyces cerevisiae Proteins)
EC 2.7.- (Protein Kinases)
EC 2.7.1.- (DUN1 protein, S cerevisiae)
EC 2.7.11.1 (Protein Serine-Threonine Kinases)
EC 3.1.3.2 (Phosphoric Monoester Hydrolases) - Publication Date: Date Created: 20020124 Date Completed: 20020214 Latest Revision: 20230323
- Publication Date: 20240104
- Accession Number: 10.1038/415180a
- Accession Number: 11805837
- Source:
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