Validation of conductance catheter system for quantification of murine pressure-volume loops.

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  • Author(s): Yang B;Yang B; Larson DF; Beischel J; Kelly R; Shi J; Watson RR
  • Source:
    Journal of investigative surgery : the official journal of the Academy of Surgical Research [J Invest Surg] 2001 Nov-Dec; Vol. 14 (6), pp. 341-55.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: United States NLM ID: 8809255 Publication Model: Print Cited Medium: Print ISSN: 0894-1939 (Print) Linking ISSN: 08941939 NLM ISO Abbreviation: J Invest Surg Subsets: MEDLINE
    • Publication Information:
      Publication: [Philadelphia, PA] : Taylor & Francis
      Original Publication: New York : Taylor & Francis, c1988-
    • Subject Terms:
      Hemodynamics*; Cardiac Catheterization/*instrumentation; Animals ; Calibration ; Cardiac Volume ; Electric Conductivity ; Female ; Male ; Mice ; Mice, Inbred C57BL ; Ventricular Function
    • Abstract:
      The purpose of this study was to define the validation methods and outcomes of a conductance catheter system specifically for in vivo murine cardiac hemodynamic analysis. To express the relationship between conductance and blood volumes, we used an in vitro model to derive a volume-conductance line. The volume-conductance line was used to compute raw volume from the modified conductance signals. The parallel volume was calibrated with hypertonic (15%) saline injected from extrajugular vein. The ventricular volume was computed by raw volume minus parallel volume. The accuracy of conductance volumetric measurements was validated with a static in situ infusion of calibrated volumes of whole blood injected into arrested left ventricles. In vivo dynamic measurements were performed with 24 C57B1/6 mice, 6 months old; for comparison of established values. The in situ model showed that after calibration, the experimental coefficient, alpha, was equal to 1 and the measured volume by conductance catheter was equal to the true volume of the left ventricle (y = 0.982x + 0.513, p < .0001). For the in vivo models, the end-diastolic volumes and the stroke volumes and cardiac output determined with the conductance catheter system were 17.3 +/- 1.0 microL, 10.6 +/- 0.9 microL, and 6.0 +/- 0.5 mL/min, respectively. We validated the relationship between measured volume by conductance catheter and the true volume and demonstrated the accuracy of the volume-conductance line for conversion of conductance to volume.
    • Grant Information:
      HL59794-01 United States HL NHLBI NIH HHS; HL63667-01 United States HL NHLBI NIH HHS
    • Publication Date:
      Date Created: 20020322 Date Completed: 20020823 Latest Revision: 20191105
    • Publication Date:
      20240104
    • Accession Number:
      10.1080/089419301753435710
    • Accession Number:
      11905502