Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2001-
    • Subject Terms:
      Antigens, Protozoan/*immunology ; Cytokines/*biosynthesis ; Leishmania braziliensis/*immunology ; Leishmaniasis, Diffuse Cutaneous/*immunology ; Leishmaniasis, Diffuse Cutaneous/*parasitology ; Leukocytes, Mononuclear/*immunology; Adolescent ; Adult ; Animals ; Antigens, Protozoan/pharmacology ; Cells, Cultured ; Child ; Cytokines/drug effects ; Cytokines/immunology ; Enzyme-Linked Immunosorbent Assay ; Gene Expression Regulation ; Humans ; Interferon-gamma/analysis ; Interferon-gamma/biosynthesis ; Interleukin-10/analysis ; Interleukin-10/biosynthesis ; Interleukin-5/analysis ; Interleukin-5/biosynthesis ; Leishmaniasis, Cutaneous/immunology ; Leishmaniasis, Cutaneous/parasitology ; Leukocytes, Mononuclear/drug effects ; Middle Aged ; Tumor Necrosis Factor-alpha/analysis ; Tumor Necrosis Factor-alpha/biosynthesis
    • Abstract:
      Background: Disseminated leishmaniasis is an emerging infectious disease, mostly due to L. braziliensis, which has clinical and histopathological features distinct from cutaneous leishmaniasis.
      Methods: In the current study we evaluated the in vitro production of the cytokines IFN-gamma, TNF-alpha, IL-5 and IL-10 by peripheral blood mononuclear cells (PBMC) from 15 disseminated leishmaniasis and 24 cutaneous leishmaniasis patients upon stimulation with L. braziliensis antigens genotyped as disseminated leishmaniasis or cutaneous leishmaniasis isolates.
      Results: Regardless of the source of L. braziliensis antigens, PBMC from cutaneous leishmaniasis patients produced significantly higher IFN-gamma than PBMC from disseminated leishmaniasis patients. Levels of TNF-alpha by PBMC from cutaneous leishmaniasis patients were significantly higher than disseminated leishmaniasis patients only when stimulated by genotyped cutaneous leishmaniasis antigens. The levels of IL-5 and IL-10 production by PBMC were very low and similar in PBMCs from both disseminated leishmaniasis and cutaneous leishmaniasis patients. The immune response of each patient evaluated by the two L. braziliensis antigens was assessed in a paired analysis in which we showed that L. braziliensis genotyped as disseminated leishmaniasis isolate was more potent than L. braziliensis genotyped as cutaneous leishmaniasis isolate in triggering IFN-gamma and TNF-alpha production in both diseases and IL-5 only in cutaneous leishmaniasis patients.
      Conclusion: This study provides evidence that antigens prepared from genotypically distinct strains of L. braziliensis induce different degrees of immune response. It also indicates that both parasite and host play a role in the outcome of L. braziliensis infection.
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    • Accession Number:
      0 (Antigens, Protozoan)
      0 (Cytokines)
      0 (Interleukin-5)
      0 (Tumor Necrosis Factor-alpha)
      130068-27-8 (Interleukin-10)
      82115-62-6 (Interferon-gamma)
    • Publication Date:
      Date Created: 20060428 Date Completed: 20060725 Latest Revision: 20211020
    • Publication Date:
      20240104
    • Accession Number:
      PMC1488856
    • Accession Number:
      10.1186/1471-2334-6-75
    • Accession Number:
      16638143