Ligands to nucleic acid-specific toll-like receptors and the onset of lupus nephritis.

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  • Additional Information
    • Source:
      Publisher: Wolters Kluwer Health, on behalf of the American Society of Nephrology Country of Publication: United States NLM ID: 9013836 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1046-6673 (Print) Linking ISSN: 10466673 NLM ISO Abbreviation: J Am Soc Nephrol Subsets: MEDLINE
    • Publication Information:
      Publication: 2023- : Hagerstown, MD : Wolters Kluwer Health, on behalf of the American Society of Nephrology
      Original Publication: Baltimore, MD : Williams & Wilkins, c1990-
    • Subject Terms:
      Adjuvants, Immunologic/*adverse effects ; Aminoquinolines/*adverse effects ; DNA, Bacterial/*adverse effects ; Interferon Inducers/*adverse effects ; Lupus Nephritis/*etiology ; Poly I-C/*adverse effects ; Toll-Like Receptors/*immunology; Animals ; Autoantibodies/immunology ; B-Lymphocytes/immunology ; Cytokines/metabolism ; Dendritic Cells/metabolism ; Female ; Imiquimod ; Immunoglobulin G/blood ; Interferon-alpha/blood ; Interleukin-12 Subunit p40/blood ; Interleukin-6/blood ; Lupus Nephritis/virology ; Lymphocyte Activation/physiology ; Mice ; Spleen/immunology
    • Abstract:
      Lupus nephritis develops from a combination of genetic and environmental factors such as microbial infection. A role for microbial nucleic acids (e.g., via nucleic acid-specific Toll-like receptors [TLR]) was hypothesized, in this context, because microbial nucleic acids can trigger multiple aspects of autoimmunity in vitro and in vivo. Eight-week-old MRL(lpr/lpr) and MRL wild-type mice received an injection of pI:C RNA (ligand to TLR-3), imiquimod (ligand to TLR-7), or CpG-DNA (ligand to TLR-9) on alternate days for 2 wk. Only CpG-DNA triggered the onset of lupus nephritis in MRL(lpr/lpr) mice, as defined by diffuse proliferative glomerulonephritis associated with glomerular IgG and complement C3 deposition, proteinuria, and glomerular macrophage infiltrates. None of the compounds caused DNA autoantibody production or glomerulonephritis in MRL wild-type mice. The role of CpG-DNA to trigger lupus nephritis in MRL(lpr/lpr) mice was found to relate to its potent immunostimulatory effects at multiple levels: B cell IL12p40 production, B cell proliferation, double-stranded DNA autoantibody secretion, and dendritic cell IFN-alpha production. The induction of lupus nephritis by CpG-DNA is motif specific and could be prevented by co-injection of inhibitory DNA. In summary, among the ligands tested, CpG-DNA triggers lupus nephritis in genetically predisposed hosts. These data support the concept that systemic lupus erythematosus is triggered by pathogens that release CG-rich DNA.
    • Comments:
      Comment in: J Am Soc Nephrol. 2006 Dec;17(12):3273-5. (PMID: 17108312)
    • Accession Number:
      0 (Adjuvants, Immunologic)
      0 (Aminoquinolines)
      0 (Autoantibodies)
      0 (CpG-DNA, E coli)
      0 (Cytokines)
      0 (DNA, Bacterial)
      0 (Immunoglobulin G)
      0 (Interferon Inducers)
      0 (Interferon-alpha)
      0 (Interleukin-12 Subunit p40)
      0 (Interleukin-6)
      0 (Toll-Like Receptors)
      O84C90HH2L (Poly I-C)
      P1QW714R7M (Imiquimod)
    • Publication Date:
      Date Created: 20061104 Date Completed: 20070227 Latest Revision: 20181201
    • Publication Date:
      20240104
    • Accession Number:
      10.1681/ASN.2006030263
    • Accession Number:
      17082246