CoCl(2)-simulated hypoxia in skeletal muscle cell lines: Role of free radicals in gene up-regulation and induction of apoptosis.

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  • Additional Information
    • Source:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 9423872 Publication Model: Print Cited Medium: Print ISSN: 1071-5762 (Print) Linking ISSN: 10292470 NLM ISO Abbreviation: Free Radic Res Subsets: MEDLINE
    • Publication Information:
      Publication: London : Informa Healthcare
      Original Publication: Yverdon, Switzerland : New York, NY : Harwood Academic ; distributed by STBS Ltd., c1994-
    • Subject Terms:
    • Abstract:
      Since it was suggested that cobalt chloride (CoCl(2)) could mimic the O(2) sensing role of mitochondria by increasing reactive oxygen species (ROS) generation during normoxia, we studied the correlation between CoCl(2)-generation of free radicals and the induction of a hypoxic cellular response in myogenic cell lines. In both L6C5 and C2C12 cell lines, exposure to CoCl(2) induced an increase of intracellular oxidants, the accumulation of HIF-1alpha protein, and the expression of vascular endothelial growth factor (VEGF) and/or iNOS genes. On the other hand, only ascorbic acid, but not trolox, was effective in lowering the CoCl(2) gene up-regulation. Neither the cytotoxicity nor the apoptosis induced by CoCl(2) in skeletal muscle cells were modified by culture supplementation with either ascorbic acid or trolox. Thus, CoCl(2) treatment of myogenic cell lines may represent a useful and convenient in vitro model to study gene modulation induced by hypoxia in skeletal muscle, although cellular loss induced by this metal may involve mechanisms other than HIF-1alpha stabilization. It is unlikely, however, that ROS would represent the main mediators of CoCl(2) effects on muscle cells.
    • Accession Number:
      0 (Free Radicals)
      0 (Hif1a protein, mouse)
      0 (Hypoxia-Inducible Factor 1, alpha Subunit)
      0 (Reactive Oxygen Species)
      0 (Vascular Endothelial Growth Factor A)
      3G0H8C9362 (Cobalt)
      EVS87XF13W (cobaltous chloride)
      S88TT14065 (Oxygen)
    • Publication Date:
      Date Created: 20070425 Date Completed: 20070612 Latest Revision: 20161124
    • Publication Date:
      20240104
    • Accession Number:
      10.1080/10715760601096799
    • Accession Number:
      17454121