A study of femoral neck fracture repair using a recombinant human bone morphogenetic protein-2 directional release system.

Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101466659 Publication Model: Print Cited Medium: Internet ISSN: 1937-335X (Electronic) Linking ISSN: 19373341 NLM ISO Abbreviation: Tissue Eng Part A Subsets: MEDLINE

Original Publication: New Rochelle, NY : Mary Ann Liebert, Inc.

Bone Morphogenetic Proteins/*administration & dosage ; Bone Morphogenetic Proteins/*pharmacology ; Femoral Neck Fractures/*pathology ; Fracture Healing/*drug effects ; Recombinant Proteins/*administration & dosage ; Recombinant Proteins/*pharmacology ; Transforming Growth Factor beta/*administration & dosage ; Transforming Growth Factor beta/*pharmacology; Animals ; Bone Morphogenetic Protein 2 ; Bone Screws ; Dogs ; Eosine Yellowish-(YS)/metabolism ; Femoral Neck Fractures/diagnostic imaging ; Femoral Neck Fractures/surgery ; Glycolates/pharmacology ; Hematoxylin/metabolism ; Humans ; Kinetics ; Lactic Acid ; Male ; Microscopy, Electron, Scanning ; Microspheres ; Polyglycolic Acid ; Polylactic Acid-Polyglycolic Acid Copolymer ; Radiography ; Radionuclide Imaging ; Staining and Labeling

Objective: Significant difficulties are caused by the delayed union of femoral neck fractures. To address this issue, we designed a new device that applies recombinant human bone morphogenetic protein-2 (rhBMP-2) to promote fracture union.
Methods: A cannulated screw with holes was used to deliver rhBMP-2 to the fracture site. Fibrin glue was used as an adhesive agent to hold rhBMP-2 in the vicinity of fracture. RhBMP-2 was protected with polylactide-glycolide acid microspheres. RhBMP-2 release was evaluated to determine the effect of the improved screws.
Result: When polylactide-glycolide acid microspheres were used, 3.65% of the rhBMP-2 was released in the first 2 h, 5.17% was released within 8 h, and 8.95% was released within 24 h. In the microsphere + fibrin glue group, 1.15% of the rhBMP-2 was released in the first 2 h, 1.75% was released within 8 h, and 6.68% was released within 24 h. Over 42 days, about 76.75% of the rhBMP-2 was released when using fibrin glue, which was lower than the amount released using microspheres alone (91.75%). In dog, a faster repair rate was observed on the side with the improved screw than on the side with traditional screw.
Conclusion: The directional release system described here can improve the process of fracture healing and is a promising technique for repairing femoral neck fracture.

0 (Bone Morphogenetic Protein 2)
0 (Bone Morphogenetic Proteins)
0 (Glycolates)
0 (Recombinant Proteins)
0 (Transforming Growth Factor beta)
0 (recombinant human bone morphogenetic protein-2)
1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
26009-03-0 (Polyglycolic Acid)
33X04XA5AT (Lactic Acid)
TDQ283MPCW (Eosine Yellowish-(YS))
YKM8PY2Z55 (Hematoxylin)

Date Created: 20091210 Date Completed: 20100203 Latest Revision: 20181201

20240104

10.1089/ten.tea.2009.0052

19995304