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In vivo evaluation of mixtures of uncultured freshly isolated adipose-derived stem cells and demineralized bone matrix for bone regeneration in a rat critically sized calvarial defect model.
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- Author(s): Rhee SC;Rhee SC; Ji YH; Gharibjanian NA; Dhong ES; Park SH; Yoon ES
- Source:
Stem cells and development [Stem Cells Dev] 2011 Feb; Vol. 20 (2), pp. 233-42. Date of Electronic Publication: 2010 Oct 12.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101197107 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8534 (Electronic) Linking ISSN: 15473287 NLM ISO Abbreviation: Stem Cells Dev Subsets: MEDLINE
- Publication Information: Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-
- Subject Terms: Bone Regeneration* ; Stem Cell Transplantation*; Adipose Tissue/*cytology ; Bone Matrix/*transplantation ; Skull/*pathology; Animals ; Antigens, CD/metabolism ; Antigens, Differentiation/metabolism ; Bone Demineralization Technique ; Bone Density ; Cell Differentiation ; Hedgehog Proteins/antagonists & inhibitors ; Hedgehog Proteins/metabolism ; Implants, Experimental ; Mice ; Mice, Nude ; Osteoblasts/pathology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; Skull/metabolism ; Skull/physiopathology ; Stromal Cells/cytology ; Tissue Scaffolds ; Veratrum Alkaloids/pharmacology
- Abstract: Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX(®)) and/or poly(70L-lactide-co-30DL-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n=10): (1) control, (2) DBM, (3) DBM + SVF, (4) DBM + PLA, and (5) DBM + PLA + SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM + SVF and DBM + PLA + SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P=0.030<0.05). The 5 groups show different amounts of filling of bone defects (control: 13.48%; DBM: 39.94%; DBM + SVF: 57.69%; DBM + PLA: 24.86%; DBM + PLA + SVF: 42.75%). Histological evaluation revealed that there was abundant new bone formation in the DBM + SVF and DBM + PLA + SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy.
- Accession Number: 0 (Antigens, CD)
0 (Antigens, Differentiation)
0 (Hedgehog Proteins)
0 (Veratrum Alkaloids)
ZH658AJ192 (cyclopamine) - Publication Date: Date Created: 20100610 Date Completed: 20110624 Latest Revision: 20121115
- Publication Date: 20240104
- Accession Number: 10.1089/scd.2009.0525
- Accession Number: 20528145
- Source:
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