Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro.

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  • Additional Information
    • Source:
      Publisher: Hindawi Pub. Co Country of Publication: United States NLM ID: 101600173 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2314-6141 (Electronic) NLM ISO Abbreviation: Biomed Res Int Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, NY : Hindawi Pub. Co.
    • Subject Terms:
    • Abstract:
      Human mesenchymal stem cells (MSCs) have an intrinsic property for homing towards tumor sites and can be used as tumor-tropic vectors for tumor therapy. But very limited studies investigated the antitumor properties of MSCs themselves. In this study we investigated the antiglioma properties of two easily accessible MSCs, namely, human adipose tissue-derived mesenchymal stem cells (ASCs) and umbilical cord-derived mesenchymal stem cells (UC-MSCs). We found (1) MSC conditioned media can significantly inhibit the growth of human U251 glioma cell line; (2) MSC conditioned media can significantly induce apoptosis in human U251 cell line; (3) real-time PCR experiments showed significant upregulation of apoptotic genes of both caspase-3 and caspase-9 and significant downregulation of antiapoptotic genes such as survivin and XIAP after MSC conditioned media induction in U 251 cells; (4) furthermore, MSCs conditioned media culture induced rapid and complete differentiation in U251 cells. These results indicate MSCs can efficiently induce both apoptosis and differentiation in U251 human glioma cell line. Whereas UC-MSCs are more efficient for apoptosis induction than ASCs, their capability of differentiation induction is not distinguishable from each other. Our findings suggest MSCs themselves have favorable antitumor characteristics and should be further explored in future glioma therapy.
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    • Accession Number:
      0 (Culture Media, Conditioned)
      EC 3.4.22.- (Caspase 3)
      EC 3.4.22.- (Caspase 9)
    • Publication Date:
      Date Created: 20140628 Date Completed: 20160502 Latest Revision: 20181202
    • Publication Date:
      20181211
    • Accession Number:
      10.1155/2014/109389
    • Accession Number:
      24971310
  • Citations
    • ABNT:
      YANG, C. et al. Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro. Biomed Research International, [s. l.], v. 2014, p. 109389, 2014. Disponível em: . Acesso em: 22 set. 2019.
    • AMA:
      Yang C, Lei D, Ouyang W, et al. Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro. Biomed Research International. 2014;2014:109389. doi:10.1155/2014/109389.
    • APA:
      Yang, C., Lei, D., Ouyang, W., Ren, J., Li, H., Hu, J., & Huang, S. (2014). Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro. Biomed Research International, 2014, 109389. https://doi.org/10.1155/2014/109389
    • Chicago/Turabian: Author-Date:
      Yang, Chao, Deqiang Lei, Weixiang Ouyang, Jinghua Ren, Huiyu Li, Jingqiong Hu, and Shiang Huang. 2014. “Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines in Vitro.” Biomed Research International 2014: 109389. doi:10.1155/2014/109389.
    • Harvard:
      Yang, C. et al. (2014) ‘Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro’, Biomed Research International, 2014, p. 109389. doi: 10.1155/2014/109389.
    • Harvard: Australian:
      Yang, C, Lei, D, Ouyang, W, Ren, J, Li, H, Hu, J & Huang, S 2014, ‘Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro’, Biomed Research International, vol. 2014, p. 109389, viewed 22 September 2019, .
    • MLA:
      Yang, Chao, et al. “Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines in Vitro.” Biomed Research International, vol. 2014, 2014, p. 109389. EBSCOhost, doi:10.1155/2014/109389.
    • Chicago/Turabian: Humanities:
      Yang, Chao, Deqiang Lei, Weixiang Ouyang, Jinghua Ren, Huiyu Li, Jingqiong Hu, and Shiang Huang. “Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines in Vitro.” Biomed Research International 2014 (2014): 109389. doi:10.1155/2014/109389.
    • Vancouver/ICMJE:
      Yang C, Lei D, Ouyang W, Ren J, Li H, Hu J, et al. Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro. Biomed Research International [Internet]. 2014 [cited 2019 Sep 22];2014:109389. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=24971310