Platelet-rich plasma and skeletal muscle healing: a molecular analysis of the early phases of the regeneration process in an experimental animal model.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Platelet-Rich Plasma*; Muscle, Skeletal/*drug effects ; Muscle, Skeletal/*physiology ; Regeneration/*drug effects ; Wound Healing/*drug effects; Animals ; Interleukin-1beta/metabolism ; Male ; Models, Animal ; Muscle, Skeletal/injuries ; Rats ; Rats, Wistar ; Transforming Growth Factor beta1/metabolism

Platelet-rich plasma (PRP) has received increasing interest in applied medicine, being widely used in clinical practice with the aim of stimulating tissue healing. Despite the reported clinical success, there is still a lack of knowledge when considering the biological mechanisms at the base of the activity of PRP during the process of muscle healing. The aim of the present study was to verify whether the local delivery of PRP modulates specific molecular events involved in the early stages of the muscle regeneration process. The right flexor sublimis muscle of anesthetized Wistar rats was mechanically injured and either treated with PRP or received no treatment. At day 2 and 5 after surgery, the animals were sacrificed and the muscle samples evaluated at molecular levels. PRP treatment increased significantly the mRNA level of the pro-inflammatory cytokines IL-1β, and TGF-β1. This phenomenon induced an increased expression at mRNA and/or protein levels of several myogenic regulatory factors such as MyoD1, Myf5 and Pax7, as well as the muscular isoform of insulin-like growth factor1 (IGF-1Eb). No effect was detected with respect to VEGF-A expression. In addition, PRP application modulated the expression of miR-133a together with its known target serum response factor (SRF); increased the phosphorylation of αB-cristallin, with a significant improvement in several apoptotic parameters (NF-κB-p65 and caspase 3), indexes of augmented cell survival. The results of the present study indicates that the effect of PRP in skeletal muscle injury repair is due both to the modulation of the molecular mediators of the inflammatory and myogenic pathways, and to the control of secondary pathways such as those regulated by myomiRNAs and heat shock proteins, which contribute to proper and effective tissue regeneration.

JAMA. 2010 Jan 13;303(2):144-9. (PMID: 20068208)
Knee Surg Sports Traumatol Arthrosc. 2010 Apr;18(4):472-9. (PMID: 19838676)
Disabil Rehabil. 2008;30(20-22):1584-9. (PMID: 18608363)
In Vivo. 2009 Jul-Aug;23(4):567-75. (PMID: 19567392)
Mol Cell Biol. 1996 Nov;16(11):6065-74. (PMID: 8887636)
Mol Cell Endocrinol. 2006 Jun 27;252(1-2):224-30. (PMID: 16644098)
J Cell Mol Med. 2010 Oct;14(10):2495-505. (PMID: 19754672)
Int J Sports Med. 2004 Nov;25(8):582-7. (PMID: 15532000)
Injury. 2009 Aug;40(8):801-5. (PMID: 18703188)
Nucleic Acids Res. 2006;34(20):5863-71. (PMID: 17062625)
Genes Dev. 2006 Jun 15;20(12):1545-56. (PMID: 16778073)
Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1206-10. (PMID: 14745025)
Am J Sports Med. 2007 Feb;35(2):245-51. (PMID: 17099241)
Implant Dent. 2001;10(4):225-8. (PMID: 11813662)
J Biol Chem. 2003 Mar 7;278(10):8826-36. (PMID: 12477723)
Mol Med. 2009 May-Jun;15(5-6):127-35. (PMID: 19295919)
Neuromuscul Disord. 1995 Nov;5(6):501-9. (PMID: 8580732)
Biochim Biophys Acta. 2011 Aug;1813(8):1532-42. (PMID: 21640763)
Int J Sports Med. 2004 Nov;25(8):588-93. (PMID: 15532001)
J Biol Chem. 2002 Oct 11;277(41):38731-6. (PMID: 12140279)
FASEB J. 2007 May;21(7):1393-402. (PMID: 17264161)
Science. 1996 Jul 19;273(5273):359-61. (PMID: 8662523)
Trends Biotechnol. 2009 Mar;27(3):158-67. (PMID: 19187989)
Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12283-8. (PMID: 11593045)
Arthroscopy. 2012 Jul;28(7):998-1009. (PMID: 22738751)
Injury. 2009 Jun;40(6):598-603. (PMID: 19380129)
Clin J Sport Med. 2014 Mar;24(2):150-2. (PMID: 23917731)
Cell Death Differ. 2006 May;13(5):759-72. (PMID: 16410803)
Tissue Eng Part C Methods. 2009 Dec;15(4):625-34. (PMID: 19231923)
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8206-10. (PMID: 3022285)
Ann Acad Med Singap. 2009 Aug;38(8):733-4. (PMID: 19736581)
Cell Struct Funct. 2008;33(2):163-9. (PMID: 18827405)
N Engl J Med. 1994 Nov 10;331(19):1286-92. (PMID: 7935686)
Exp Cell Res. 2001 Jun 10;266(2):213-21. (PMID: 11399049)
J Biol Chem. 2000 Jan 14;275(2):1095-104. (PMID: 10625651)
J Hand Surg Am. 2003 Mar;28(2):272-8. (PMID: 12671860)
PLoS One. 2012;7(7):e41478. (PMID: 22911796)
Acta Orthop Belg. 2013 Feb;79(1):10-5. (PMID: 23547508)
Acta Orthop. 2006 Oct;77(5):806-12. (PMID: 17068715)
Br J Sports Med. 2006 Jan;40(1):40-4. (PMID: 16371489)
Int J Dev Biol. 2002;46(4):559-67. (PMID: 12141444)
Arch Orthop Trauma Surg. 2011 Mar;131(3):311-7. (PMID: 20714903)
J Muscle Res Cell Motil. 2003;24(7):421-9. (PMID: 14677645)
Appl Physiol Nutr Metab. 2006 Dec;31(6):773-81. (PMID: 17213899)
J Mol Cell Cardiol. 2003 Jun;35(6):577-93. (PMID: 12788374)
Clin Genet. 2000 Jan;57(1):16-25. (PMID: 10733231)
Blood Transfus. 2014 Jan;12 Suppl 1:s221-8. (PMID: 23867182)
J Biol Chem. 1993 Nov 15;268(32):24210-4. (PMID: 8226968)
Nat Immunol. 2002 Mar;3(3):221-7. (PMID: 11875461)
J Biol Chem. 1998 Oct 23;273(43):28346-54. (PMID: 9774459)
J Biol Chem. 1994 Aug 12;269(32):20780-4. (PMID: 8051180)
Physiol Rev. 2004 Jan;84(1):209-38. (PMID: 14715915)
Dev Dyn. 1996 Sep;207(1):75-88. (PMID: 8875078)
Curr Rev Musculoskelet Med. 2008 Dec;1(3-4):165-74. (PMID: 19468902)
Dev Dyn. 1997 Jan;208(1):75-84. (PMID: 8989522)
Am J Sports Med. 2006 Nov;34(11):1774-8. (PMID: 16735582)
J Cell Biol. 2006 Aug 28;174(5):677-87. (PMID: 16923828)
FASEB J. 2002 Oct;16(12):1630-2. (PMID: 12207010)
J Histochem Cytochem. 2001 Aug;49(8):989-1001. (PMID: 11457927)
Am J Sports Med. 2009 Jun;37(6):1135-42. (PMID: 19282509)
Endocr Rev. 1996 Oct;17(5):481-517. (PMID: 8897022)
J Cell Biol. 2010 Sep 6;190(5):867-79. (PMID: 20819939)
Am J Sports Med. 2005 Jan;33(1):43-51. (PMID: 15610998)
J Oral Maxillofac Surg. 2004 Apr;62(4):489-96. (PMID: 15085519)
J Bone Joint Surg Br. 2000 Jan;82(1):131-7. (PMID: 10697329)
Dev Biol. 2000 Feb 15;218(2):146-60. (PMID: 10656759)
Hum Mol Genet. 2011 Feb 15;20(4):790-805. (PMID: 21118895)
EMBO J. 1996 Jun 3;15(11):2695-706. (PMID: 8654367)
Nature. 2005 Jul 14;436(7048):214-20. (PMID: 15951802)
Nat Methods. 2004 Oct;1(1):47-53. (PMID: 15782152)
Br J Sports Med. 2009 Apr;43(4):247-52. (PMID: 19244270)
Circ Res. 2003 Feb 7;92(2):130-2. (PMID: 12574138)
Biochim Biophys Acta. 2008 Nov;1779(11):682-91. (PMID: 18381085)
Br J Pharmacol. 2007 Jan;150(2):176-85. (PMID: 17179954)
Arthroscopy. 2008 Dec;24(12):1373-80. (PMID: 19038708)
Wound Repair Regen. 2012 Sep-Oct;20(5):748-56. (PMID: 22882470)
Acta Orthop Scand. 2004 Feb;75(1):93-9. (PMID: 15022816)
Bull World Health Organ. 2003;81(9):646-56. (PMID: 14710506)
Nat Med. 2007 May;13(5):613-8. (PMID: 17468766)
Am J Physiol Cell Physiol. 2007 Jul;293(1):C451-7. (PMID: 17459947)
Knee Surg Sports Traumatol Arthrosc. 2011 Apr;19(4):516-27. (PMID: 21082164)
Knee Surg Sports Traumatol Arthrosc. 2011 Apr;19(4):528-35. (PMID: 20740273)
Am J Pathol. 2003 May;162(5):1685-91. (PMID: 12707053)
J Cell Biol. 2006 Oct 9;175(1):77-85. (PMID: 17030984)
J Biol Chem. 2005 Nov 4;280(44):37139-48. (PMID: 16129694)
Trends Cell Biol. 2006 Jan;16(1):36-44. (PMID: 16325404)
Stem Cells. 1999;17(3):127-37. (PMID: 10342555)
Nat Genet. 2006 Feb;38(2):228-33. (PMID: 16380711)
Int J Artif Organs. 1987 Jan;10(1):47-50. (PMID: 3570542)
Am J Physiol Cell Physiol. 2008 Feb;294(2):C467-76. (PMID: 18077604)
J Appl Physiol (1985). 2007 Jan;102(1):306-13. (PMID: 17008435)
PLoS One. 2013 Apr 23;8(4):e62687. (PMID: 23626847)
Prog Clin Biol Res. 1988;266:319-29. (PMID: 3289047)
Cell Mol Life Sci. 2009 Oct;66(20):3289-307. (PMID: 19593530)
Endocrinology. 2010 Mar;151(3):865-75. (PMID: 20130113)
Nat Cell Biol. 2007 Mar;9(3):255-67. (PMID: 17293855)

0 (Interleukin-1beta)
0 (Transforming Growth Factor beta1)

Date Created: 20140724 Date Completed: 20151120 Latest Revision: 20220331

20240104

PMC4108405

10.1371/journal.pone.0102993

25054279