In vitro induction of human adipose-derived stem cells into lymphatic endothelial-like cells.

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  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101528176 Publication Model: Print Cited Medium: Internet ISSN: 2152-4998 (Electronic) Linking ISSN: 21524971 NLM ISO Abbreviation: Cell Reprogram Subsets: MEDLINE
    • Publication Information:
      Original Publication: New Rochelle, NY : Mary Ann Liebert, Inc.
    • Subject Terms:
    • Abstract:
      Human adipose-derived stem cells (hADSCs) may provide a suitable number of progenitors for the treatment of lymphatic edema; however, to date the protocols for inducing hADSCs into this tissue type have not been standardized. We wished to investigate the induction of hADSCs into lymphatic endothelial-like cells using vascular endothelial growth factor-C156S (VEGF-C156S) and other growth factors in vitro. hADSCs from healthy adult adipose tissue were purified using enzyme digestion. Differentiation was induced using medium containing VEGF-C156S and bovine fibroblast growth factor (bFGF). Differentiation was confirmed using immunostaining for lymphatic vessel endothelial hyaluronan receptor (LYVE-1) and fms-related tyrosine kinase 4 (FLT-4), two lymphatic endothelial cell markers. The expression levels of LYVE-1, prospero homeobox 1 (PROX-1), and FLT-4 throughout induction were assessed using reverse transcriptase quantitative polymerase chain reaction. hADSCs were successfully obtained by trypsin digest and purification. Flow cytometry showed these cells were similar to mesenchymal stem cells, with a high positive rate of CD13, CD29, CD44, and CD105, and a low positive rate of CD31, CD34, CD45, and HLA-DR. Induction to lymphatic endothelial-like cells was successful, with cells expressing high levels of LYVE-1, PROX-1, and FLT-4. Adipose-derived stem cells can be induced to differentiate into lymphatic endothelial-like cells using a medium containing VEGF-C156S, bFGF, and other growth factors. This population of lymphatic endothelial-like cells may be useful for lymphatic reconstruction in the future.
    • Accession Number:
      0 (Antigens, CD)
      0 (Homeodomain Proteins)
      0 (LYVE1 protein, human)
      0 (Tumor Suppressor Proteins)
      0 (Vascular Endothelial Growth Factor C)
      0 (Vesicular Transport Proteins)
      0 (prospero-related homeobox 1 protein)
      EC 2.7.10.1 (FLT4 protein, human)
      EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-3)
    • Publication Date:
      Date Created: 20150204 Date Completed: 20150723 Latest Revision: 20161125
    • Publication Date:
      20220302
    • Accession Number:
      10.1089/cell.2014.0043
    • Accession Number:
      25647247