Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity.

Publisher: Hindawi Publishing Corporation Country of Publication: Egypt NLM ID: 101627166 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2314-7156 (Electronic) Linking ISSN: 23147156 NLM ISO Abbreviation: J Immunol Res Subsets: MEDLINE

Original Publication: Cairo, Egypt : Hindawi Publishing Corporation, [2014]-

Antibodies, Protozoan/*immunology ; Antibody Affinity/*immunology ; Antigens, Protozoan/*immunology ; Plasmodium/*immunology; Adjuvants, Immunologic ; Animals ; Antigens, Protozoan/isolation & purification ; Disease Models, Animal ; Female ; Immunization ; Immunoglobulin G/immunology ; Malaria/immunology ; Malaria/prevention & control ; Malaria Vaccines/immunology ; Mice

Vaccines blocking malaria parasites in the blood-stage diminish mortality and morbidity caused by the disease. Here, we isolated antigens from total parasite proteins by antibody affinity chromatography to test an immunization against lethal malaria infection in a murine model. We used the sera of malaria self-resistant ICR mice to lethal Plasmodium yoelii yoelii 17XL for purification of their IgGs which were subsequently employed to isolate blood-stage parasite antigens that were inoculated to immunize BALB/c mice. The presence of specific antibodies in vaccinated mice serum was studied by immunoblot analysis at different days after vaccination and showed an intensive immune response to a wide range of antigens with molecular weight ranging between 22 and 250 kDa. The humoral response allowed delay of the infection after the inoculation to high lethal doses of P. yoelii yoelii 17XL resulting in a partial protection against malaria disease, although final survival was managed in a low proportion of challenged mice. This approach shows the potential to prevent malaria disease with a set of antigens isolated from blood-stage parasites.

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0 (Adjuvants, Immunologic)
0 (Antibodies, Protozoan)
0 (Antigens, Protozoan)
0 (Immunoglobulin G)
0 (Malaria Vaccines)

Date Created: 20151106 Date Completed: 20160819 Latest Revision: 20181113

20240104

PMC4619943

10.1155/2015/723946

26539558