Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Gene Expression Regulation, Developmental*; Carrier Proteins/*genetics ; Germ Cells/*metabolism ; Proteins/*genetics ; Spermatids/*metabolism ; Spermatogenesis/*genetics; Amino Acid Sequence ; Animals ; Argonaute Proteins/genetics ; Argonaute Proteins/metabolism ; Carrier Proteins/metabolism ; Cell Line ; Cells, Cultured ; Female ; Fluorescent Antibody Technique ; Germ Cells/growth & development ; Immunoblotting ; Male ; Mice, 129 Strain ; Mice, Inbred C57BL ; Mice, Knockout ; Molecular Sequence Data ; Protein Binding ; Proteins/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Spermatids/growth & development ; Testis/cytology ; Testis/metabolism

The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice.

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0 (Argonaute Proteins)
0 (Carrier Proteins)
0 (GTSF1L protein, mouse)
0 (GTSF2 protein, mouse)
0 (Proteins)

Date Created: 20160302 Date Completed: 20160802 Latest Revision: 20190222

20240104

PMC4773171

10.1371/journal.pone.0150390

26930067