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Numb Gene Enhances Radiation Sensitivity of Nonsmall Cell Lung Cancer Stem Cells.
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- Author(s): Shan GP;Shan GP; Zhang P; Zhang P; Li P; Li P; Du FL; Du FL; Yang YW; Yang YW
- Source:
Cancer biotherapy & radiopharmaceuticals [Cancer Biother Radiopharm] 2016 Jun; Vol. 31 (5), pp. 180-8.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9605408 Publication Model: Print Cited Medium: Internet ISSN: 1557-8852 (Electronic) Linking ISSN: 10849785 NLM ISO Abbreviation: Cancer Biother Radiopharm Subsets: MEDLINE
- Publication Information: Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1996-
- Subject Terms: Carcinoma, Non-Small-Cell Lung/*genetics ; Carcinoma, Non-Small-Cell Lung/*radiotherapy ; Lung Neoplasms/*genetics ; Lung Neoplasms/*radiotherapy ; Membrane Proteins/*genetics ; Nerve Tissue Proteins/*genetics; Apoptosis/genetics ; Apoptosis/radiation effects ; Carcinoma, Non-Small-Cell Lung/metabolism ; Carcinoma, Non-Small-Cell Lung/pathology ; Cell Line, Tumor ; Cell Proliferation/genetics ; Cell Proliferation/radiation effects ; Humans ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; Membrane Proteins/biosynthesis ; Membrane Proteins/metabolism ; Neoplastic Stem Cells/radiation effects ; Nerve Tissue Proteins/biosynthesis ; Nerve Tissue Proteins/metabolism ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; Radiation Tolerance ; Transfection
- Abstract: Objective: To study the effects of Numb gene expression on radiation sensitivity of nonsmall cell lung cancer (NSCLC) stem cells.
Materials and Methods: The side population (SP) cells A549-SP were transfected with pcDNA3.1 (pcDNA3.1 group), pcDNA-Numb (pcDNA-Numb group) and shRNA-Numb (shRNA-Numb group). Real-time quantitative polymerase chain reaction and Western blot were performed to determine Numb expression; MTT method was used to measure the proliferation activity change of the NSCLC stem cells both before and after irradiation with different doses of 60Coγ ray; Hoechst staining and Annexin V-FITC/PI were used to detect the apoptosis of the NSCLC stem cells; and colony-forming assay was used to determine the effect of Numb expression on radiation sensitivity of the NSCLC stem cells.
Results: Increased mRNA and protein expressions of the A549-SP cells were found in the pcDNA-Numb group, and decreased mRNA and protein expressions were found in the shRNA-Numb group. The optical density value of the cells decreased in the pcDNA-Numb group but increased in the shRNA-Numb group. The cells with over-expressed Numb showed obvious nuclear condensation and fragmentation; the apoptosis rate increased significantly. The cells with knockdown Numb showed less nuclear damage; the apoptosis rate significantly decreased. After irradiation, the cells in the pcDNA-Numb group showed decreased survival rate, clonality, and the values of D0, Dq, N, and SF2; whereas the cells in the shRNA-Numb group showed the opposite trend.
Conclusions: Radiation sensitivity of NSCLC stem cells was enhanced with the increase of Numb expression. Determination of Numb expression helped to evaluate the response of lung cancer to radiotherapy, which was important for guiding tumor treatment clinically. - Contributed Indexing: Keywords: Numb; gene silencing; nonsmall cell lung cancer stem cells; over-expression; radiation sensitivity; stem cell apoptosis; stem cell proliferation; tumor biology
- Accession Number: 0 (Membrane Proteins)
0 (Nerve Tissue Proteins)
0 (NUMB protein, human)
0 (RNA, Messenger) - Publication Date: Date Created: 20160617 Date Completed: 20170209 Latest Revision: 20231213
- Publication Date: 20240104
- Accession Number: 10.1089/cbr.2016.2053
- Accession Number: 27310305
- Source:
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