Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101088663 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6750 (Electronic) Linking ISSN: 14726750 NLM ISO Abbreviation: BMC Biotechnol. Subsets: MEDLINE
    • Publication Information:
      Original Publication: [London] : BioMed Central, 2001-
    • Subject Terms:
    • Abstract:
      Background: Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported as an efficient method for producing transgenic chickens, the inefficiency of cBCs could potentially be resolved by inducing them to differentiate into germ cells. However, whether chemical inducers are able to enhance cBCs germline competence in vitro is unknown and the molecular mechanisms of differentiation of chicken pluripotent cells into germ cells are poorly understood.
      Results: We cultured cBCs with a monolayer morphology in E8 medium, a xeno- and feeder-free medium. We showed that retinoic acid (RA) treatment increased expression of germ cell-specific genes in cBCs. Using western blot, we determined that RA stimulated Smad1/5 phosphorylation. Moreover, Smad1/5 activation regulates the expression of germ cell-specific genes, as co-treatment with a Smad1/5 phosphorylation inhibitor or activator alters expression of these genes. We also demonstrate that Smad1/5 is required for RA-induced differentiation by RNA interference knockdown.
      Conclusion: Our results demonstrated that E8 medium is able to maintain cBC growth for weeks and RA treatment induced germ cell differentiation of cBCs through the BMP-Smad1/5 signaling pathway.
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    • Contributed Indexing:
      Keywords: Chicken blastodermal cells*; E8 medium*; Germ cell differentiation*; Growth factor*; Retinoic acid*; Smad1/5 phosphorylation*
    • Accession Number:
      0 (Smad1 Protein)
      0 (Smad5 Protein)
      5688UTC01R (Tretinoin)
    • Publication Date:
      Date Created: 20170222 Date Completed: 20170613 Latest Revision: 20181113
    • Publication Date:
      20200527
    • Accession Number:
      PMC5319176
    • Accession Number:
      10.1186/s12896-017-0332-y
    • Accession Number:
      28219352
  • Citations
    • ABNT:
      TANG, X. et al. Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system. BMC biotechnology, [s. l.], v. 17, n. 1, p. 17, 2017. DOI 10.1186/s12896-017-0332-y. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=28219352. Acesso em: 3 jul. 2020.
    • AMA:
      Tang X, Xu S, Zhang H, et al. Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system. BMC biotechnology. 2017;17(1):17. doi:10.1186/s12896-017-0332-y.
    • AMA11:
      Tang X, Xu S, Zhang H, et al. Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system. BMC biotechnology. 2017;17(1):17. doi:10.1186/s12896-017-0332-y
    • APA:
      Tang, X., Xu, S., Zhang, H., Chen, Q., Li, R., Wu, W., Yu, M., & Liu, H. (2017). Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system. BMC Biotechnology, 17(1), 17. https://doi.org/10.1186/s12896-017-0332-y
    • Chicago/Turabian: Author-Date:
      Tang, Xiaochuan, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu, and Honglin Liu. 2017. “Retinoic Acid Promotes Expression of Germline-Specific Genes in Chicken Blastoderm Cells by Stimulating Smad1/5 Phosphorylation in a Feeder-Free Culture System.” BMC Biotechnology 17 (1): 17. doi:10.1186/s12896-017-0332-y.
    • Harvard:
      Tang, X. et al. (2017) ‘Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system’, BMC biotechnology, 17(1), p. 17. doi: 10.1186/s12896-017-0332-y.
    • Harvard: Australian:
      Tang, X, Xu, S, Zhang, H, Chen, Q, Li, R, Wu, W, Yu, M & Liu, H 2017, ‘Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system’, BMC biotechnology, vol. 17, no. 1, p. 17, viewed 3 July 2020, .
    • MLA:
      Tang, Xiaochuan, et al. “Retinoic Acid Promotes Expression of Germline-Specific Genes in Chicken Blastoderm Cells by Stimulating Smad1/5 Phosphorylation in a Feeder-Free Culture System.” BMC Biotechnology, vol. 17, no. 1, Feb. 2017, p. 17. EBSCOhost, doi:10.1186/s12896-017-0332-y.
    • Chicago/Turabian: Humanities:
      Tang, Xiaochuan, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu, and Honglin Liu. “Retinoic Acid Promotes Expression of Germline-Specific Genes in Chicken Blastoderm Cells by Stimulating Smad1/5 Phosphorylation in a Feeder-Free Culture System.” BMC Biotechnology 17, no. 1 (February 20, 2017): 17. doi:10.1186/s12896-017-0332-y.
    • Vancouver/ICMJE:
      Tang X, Xu S, Zhang H, Chen Q, Li R, Wu W, et al. Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system. BMC biotechnology [Internet]. 2017 Feb 20 [cited 2020 Jul 3];17(1):17. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=28219352