Assessing the effectiveness of mitochondrial COI and 16S rRNA genes for DNA barcoding of farmland spiders in China.

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  • Author(s): Wang ZL;Wang ZL; Yang XQ; Yang XQ; Wang TZ; Wang TZ; Yu X; Yu X
  • Source:
    Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis [Mitochondrial DNA A DNA Mapp Seq Anal] 2018 Jul; Vol. 29 (5), pp. 695-702. Date of Electronic Publication: 2017 Jul 15.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Taylor & Francis Group Country of Publication: England NLM ID: 101679980 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2470-1408 (Electronic) Linking ISSN: 24701394 NLM ISO Abbreviation: Mitochondrial DNA A DNA Mapp Seq Anal Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Abingdon] : Taylor & Francis Group, [2016]-
    • Subject Terms:
      Electron Transport Complex IV/*genetics ; Genome, Insect/*genetics ; Spiders/*genetics; Animals ; China ; DNA ; DNA Barcoding, Taxonomic/methods ; Farms ; Genetic Speciation ; Genetic Variation ; Genome, Mitochondrial ; Mitochondria/genetics ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Species Specificity
    • Abstract:
      DNA barcoding has been widely used to identify and discover new species in a wide range of taxa. In order to assess the effectiveness of COI (cytochrome C oxidase subunit I) and 16S (16S ribosomal RNA) in the discrimination of spiders, we have generated 289 barcodes for a total of 56 farmland spider species from 14 different families for the first time in China. Our results reveal that the standard barcoding marker COI can be used to distinguish the farmland spiders both in species and family level by NJ tree-based method, despite the absence of a barcode gap between the intra- and inter-specific genetic divergences. 16S has a lower species identification success as compared with COI. However, almost 98% of the species can be correctly distinguished for both COI and 16S when a threshold of 3% nucleotide divergence was used for species discrimination. Our study significantly improves the barcode reference sequence library for Chinese farmland spiders, and will be very useful in pest management and eco-environmental monitoring and protection.
    • Contributed Indexing:
      Keywords: 16S rRNA; COI; DNA barcoding; spider
    • Accession Number:
      0 (RNA, Ribosomal, 16S)
      9007-49-2 (DNA)
      EC 1.9.3.1 (Electron Transport Complex IV)
    • Publication Date:
      Date Created: 20170718 Date Completed: 20181219 Latest Revision: 20181219
    • Publication Date:
      20240105
    • Accession Number:
      10.1080/24701394.2017.1350949
    • Accession Number:
      28712321