Pendimethalin induces oxidative stress, DNA damage, and mitochondrial dysfunction to trigger apoptosis in human lymphocytes and rat bone-marrow cells.

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  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: Germany NLM ID: 9506663 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-119X (Electronic) Linking ISSN: 09486143 NLM ISO Abbreviation: Histochem Cell Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin : Springer,
    • Subject Terms:
      Aniline Compounds/*pharmacology ; Apoptosis/*drug effects ; Bone Marrow Cells/*drug effects ; Lymphocytes/*drug effects ; Mitochondria/*drug effects ; Oxidative Stress/*drug effects; Aniline Compounds/chemistry ; Animals ; Bone Marrow Cells/metabolism ; DNA Damage ; Humans ; Lymphocytes/metabolism ; Male ; Mitochondria/metabolism ; Molecular Docking Simulation ; Rats ; Rats, Wistar
    • Abstract:
      Pendimethalin (PM) is a dinitroaniline herbicide extensively applied against the annual grasses and broad-leaved weeds. There is no report available on PM-induced low-dose genotoxicity in human primary cells and in vivo test models. Such data gap has prompted us to evaluate the genotoxic potential of PM in human lymphocytes and rats. PM selectively binds in the minor groove of DNA by forming covalent bonds with G and C nitrogenous bases, as well as with the ribose sugar. PM induces micronucleus formation (MN) in human lymphocytes, indicating its clastogenic potential. Comet assay data showed 35.6-fold greater DNA damage in PM (200 μM)-treated human lymphocytes. Rat bone-marrow cells, at the highest dose of 50 mg/kg b w/day of PM also exhibited 10.5-fold greater DNA damage. PM at 200 μM and 50 mg/kg b w/day induces 193.4 and 229% higher reactive oxygen species generation in human lymphocytes and rat bone-marrow cells. PM-treated human lymphocytes and rat bone-marrow cells both showed dysfunction of mitochondrial membrane potential (ΔΨ m ). PM exposure results in the appearance of 72.2 and 35.2% sub-G 1 apoptotic peaks in human lymphocytes and rat bone-marrow cells when treated with 200 μM and 50 mg/kg b w/day of PM. Rats exposed to PM also showed imbalance in antioxidant enzymes and histological pathology. Overall, our data demonstrated the genotoxic and apoptotic potentials of PM in human and animal test models.
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    • Contributed Indexing:
      Keywords: Apoptosis; DNA damage; Genotoxicity; Oxidative stress; Pendimethalin; Pesticide
    • Accession Number:
      0 (Aniline Compounds)
      VL6L14C06U (pendimethalin)
    • Publication Date:
      Date Created: 20171120 Date Completed: 20180516 Latest Revision: 20181113
    • Publication Date:
      20240105
    • Accession Number:
      10.1007/s00418-017-1622-0
    • Accession Number:
      29151145