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Tumor necrosis factor-α regulates human follicular dendritic cell-secreted protein gene transcription in gingival epithelial cells.
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- Author(s): Iwai Y;Iwai Y; Noda K; Noda K; Yamazaki M; Yamazaki M; Kato A; Kato A; Kato A; Mezawa M; Mezawa M; Mezawa M; Takai H; Takai H; Takai H; Nakayama Y; Nakayama Y; Nakayama Y; Ogata Y; Ogata Y; Ogata Y
- Source:
Genes to cells : devoted to molecular & cellular mechanisms [Genes Cells] 2018 Mar; Vol. 23 (3), pp. 161-171. Date of Electronic Publication: 2018 Jan 22.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Blackwell Science Ltd Country of Publication: England NLM ID: 9607379 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2443 (Electronic) Linking ISSN: 13569597 NLM ISO Abbreviation: Genes Cells Subsets: MEDLINE
- Publication Information: Original Publication: Oxford, UK : Blackwell Science Ltd., 1996-
- Subject Terms: Gene Expression Regulation* ; Transcription, Genetic*; Epithelial Cells/*metabolism ; Gingiva/*metabolism ; Proteins/*genetics ; Tumor Necrosis Factor-alpha/*metabolism; CCAAT-Enhancer-Binding Proteins/genetics ; CCAAT-Enhancer-Binding Proteins/metabolism ; Cells, Cultured ; Epithelial Cells/cytology ; GATA Transcription Factors/genetics ; GATA Transcription Factors/metabolism ; Gingiva/cytology ; Humans ; Promoter Regions, Genetic ; YY1 Transcription Factor/genetics ; YY1 Transcription Factor/metabolism
- Abstract: Follicular dendritic cell-secreted protein (FDC-SP) is a secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium. To elucidate the transcriptional regulation of the human FDC-SP gene by tumor necrosis factor-α (TNF-α), we conducted real-time PCR, Western blotting, transient transfection analyses with chimeric constructs of the FDC-SP gene promoter linked to a luciferase reporter gene, gel mobility shift and chromatin immunoprecipitation assays using Ca9-22 gingival epithelial cells. TNF-α (10 ng/ml) induced FDC-SP mRNA and protein levels at 3 hr and reached maximum at 12 hr. In transient transfection assays, TNF-α (12 hr) increased the LUC activities of constructs between -116FDCSP and -948FDCSP including the human FDC-SP gene promoter. Transcriptional stimulations by TNF-α were partially inhibited in the -345FDCSP constructs that included 3-bp mutations in the YY1, GATA, CCAAT enhancer-binding protein 2 (C/EBP2) and C/EBP3. Transcriptional activities induced by TNF-α were inhibited by tyrosine kinase, MEK1/2 and phosphoinositide 3-kinase inhibitors. The results of ChIP assays showed that YY1, GATA and C/EBPβ transcription factors interacted with the YY1, GATA, C/EBP2 and C/EBP3 elements that were increased by TNF-α. These studies show that TNF-α stimulates human FDC-SP gene transcription by targeting YY1, GATA, C/EBP2 and C/EBP3 in the FDC-SP gene promoter.
(© 2018 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.) - Contributed Indexing: Keywords: TNF-α; follicular dendritic cell secreted protein; gene promoter; inflammation; junctional epithelium; periodontitis; transcription
- Accession Number: 0 (CCAAT-Enhancer-Binding Proteins)
0 (FDCSP protein, human)
0 (GATA Transcription Factors)
0 (Proteins)
0 (Tumor Necrosis Factor-alpha)
0 (YY1 Transcription Factor)
0 (YY1 protein, human) - Publication Date: Date Created: 20180123 Date Completed: 20180925 Latest Revision: 20180925
- Publication Date: 20240104
- Accession Number: 10.1111/gtc.12561
- Accession Number: 29356241
- Source:
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