Expression of the eight GABAA receptor α subunits in the developing zebrafish central nervous system.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Central Nervous System/*growth & development ; Receptors, GABA-A/*genetics ; Receptors, GABA-A/*metabolism ; Zebrafish/*growth & development; Animals ; Brain/growth & development ; Brain/metabolism ; Central Nervous System/metabolism ; Gene Expression Regulation, Developmental ; In Situ Hybridization ; Multigene Family ; Phylogeny ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Retina/growth & development ; Retina/metabolism ; Spinal Cord/growth & development ; Spinal Cord/metabolism ; Tissue Distribution ; Zebrafish/genetics ; Zebrafish/metabolism ; Zebrafish Proteins/genetics ; Zebrafish Proteins/metabolism
    • Abstract:
      GABA is a robust regulator of both developing and mature neural networks. It exerts many of its effects through GABAA receptors, which are heteropentamers assembled from a large array of subunits encoded by distinct genes. In mammals, there are 19 different GABAA subunit types, which are divided into the α, β, γ, δ, ε, π, θ and ρ subfamilies. The immense diversity of GABAA receptors is not fully understood. However, it is known that specific isoforms, with their distinct biophysical properties and expression profiles, tune responses to GABA. Although larval zebrafish are well-established as a model system for neural circuit analysis, little is known about GABAA receptors diversity and expression in this system. Here, using database analysis, we show that the zebrafish genome contains at least 23 subunits. All but the mammalian θ and ε subunits have at least one zebrafish ortholog, while five mammalian GABAA receptor subunits have two zebrafish orthologs. Zebrafish contain one subunit, β4, which does not have a clear mammalian ortholog. Similar to mammalian GABAA receptors, the zebrafish α subfamily is the largest and most diverse of the subfamilies. In zebrafish there are eight α subunits, and RNA in situ hybridization across early zebrafish development revealed that they demonstrate distinct patterns of expression in the brain, spinal cord, and retina. Some subunits were very broadly distributed, whereas others were restricted to small populations of cells. Subunit-specific expression patterns in zebrafish resembled were those found in frogs and rodents, which suggests that the roles of different GABAA receptor isoforms are largely conserved among vertebrates. This study provides a platform to examine isoform specific roles of GABAA receptors within zebrafish neural circuits and it highlights the potential of this system to better understand the remarkable heterogeneity of GABAA receptors.
    • References:
      Curr Opin Neurobiol. 2011 Feb;21(1):110-5. (PMID: 20970321)
      Cell Mol Neurobiol. 2005 Jun;25(3-4):607-24. (PMID: 16075381)
      Genesis. 2002 Nov;34(3):196-202. (PMID: 12395384)
      Nat Methods. 2015 Nov;12(11):1039-46. (PMID: 26778924)
      Nat Protoc. 2008;3(1):59-69. (PMID: 18193022)
      Cell Tissue Res. 2006 Nov;326(2):505-16. (PMID: 16937111)
      Nat Genet. 1998 Apr;18(4):345-9. (PMID: 9537416)
      J Neurosci. 1992 Mar;12(3):1063-76. (PMID: 1312132)
      Eur J Neurosci. 2007 Jan;25(2):460-70. (PMID: 17284187)
      Neuroreport. 2014 Oct 22;25(15):1232-6. (PMID: 25162782)
      J Neurosci. 2000 May 15;20(10):3588-95. (PMID: 10804200)
      Methods. 2006 Jul;39(3):246-54. (PMID: 16870470)
      Br J Pharmacol. 2006 Jan;147 Suppl 1:S109-19. (PMID: 16402094)
      Science. 2010 Oct 29;330(6004):669-73. (PMID: 21030657)
      J Comp Neurol. 1990 Dec 15;302(3):603-16. (PMID: 1702120)
      Dev Dyn. 1995 Jul;203(3):253-310. (PMID: 8589427)
      J Biol Chem. 2012 Nov 23;287(48):40224-31. (PMID: 23038269)
      J Neurosci. 2010 Oct 13;30(41):13718-28. (PMID: 20943912)
      J Neurobiol. 1998 Dec;37(4):622-32. (PMID: 9858263)
      Dis Model Mech. 2012 Nov;5(6):773-84. (PMID: 22730455)
      Nat Rev Neurosci. 2005 Mar;6(3):215-29. (PMID: 15738957)
      Curr Pharm Des. 2013;19(24):4485-97. (PMID: 23360278)
      Front Neural Circuits. 2013 Oct 24;7:170. (PMID: 24167475)
      J Neurogenet. 2016 Jun;30(2):89-100. (PMID: 27302612)
      Front Mol Neurosci. 2008 May 30;1:7. (PMID: 18946540)
      Vis Neurosci. 2008 Mar-Apr;25(2):139-53. (PMID: 18442437)
      J Comp Neurol. 2004 Nov 29;480(1):1-18. (PMID: 15515020)
      Prog Brain Res. 2016;226:195-207. (PMID: 27323944)
      Nat Methods. 2012 Jun 17;9(7):735-42. (PMID: 22706672)
      Front Neural Circuits. 2013 Apr 23;7:71. (PMID: 23630467)
      J Neurosci. 1992 Mar;12(3):1040-62. (PMID: 1312131)
      J Comp Neurol. 2006 Apr 1;495(4):408-21. (PMID: 16485284)
      Adv Pharmacol. 2017;79:1-34. (PMID: 28528665)
      J Biol Chem. 2004 Oct 1;279(40):41422-35. (PMID: 15258161)
      Neuropharmacology. 2009 Jan;56(1):141-8. (PMID: 18760291)
      Toxicol Sci. 2006 Jul;92(1):270-8. (PMID: 16611622)
      Science. 1998 Nov 27;282(5394):1711-4. (PMID: 9831563)
      Front Neural Circuits. 2015 Nov 24;9:78. (PMID: 26635538)
      J Neurosci Methods. 2016 Feb 15;260:91-5. (PMID: 26219659)
      Mol Biol Evol. 2007 Feb;24(2):599-610. (PMID: 17135332)
      Dev Neurobiol. 2012 Mar;72(3):366-72. (PMID: 22328273)
      Neuroscience. 2005;131(3):759-68. (PMID: 15730879)
      J Comp Neurol. 1992 Dec 8;326(2):263-72. (PMID: 1479075)
      Neuroscience. 1997 Sep;80(1):133-45. (PMID: 9252227)
      Eur J Neurosci. 2014 Jun;39(11):1845-65. (PMID: 24628861)
      PLoS One. 2007 Sep 19;2(9):e894. (PMID: 17878929)
      Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1164-9. (PMID: 21199947)
      Dev Dyn. 2011 Apr;240(4):862-73. (PMID: 21384470)
      Neural Dev. 2012 Sep 18;7:32. (PMID: 22989074)
      Brain Res Rev. 2008 Jan;57(1):86-93. (PMID: 17825423)
      J Neurosci. 1992 Nov;12(11):4151-72. (PMID: 1331359)
      Nucleic Acids Res. 1994 Nov 11;22(22):4673-80. (PMID: 7984417)
      Curr Biol. 2016 Nov 7;26(21):2841-2853. (PMID: 27720623)
      Neurochem Res. 2014 Jun;39(6):1104-17. (PMID: 24352815)
      Syst Biol. 2010 May;59(3):307-21. (PMID: 20525638)
      Neuroscience. 2017 Feb 20;343 :300-321. (PMID: 27453477)
      Biochem Biophys Rep. 2015 Aug 06;3:190-201. (PMID: 29124181)
      Pharmacol Rev. 2008 Sep;60(3):243-60. (PMID: 18790874)
    • Grant Information:
      R15 DC015352 United States DC NIDCD NIH HHS
    • Accession Number:
      0 (Protein Isoforms)
      0 (Receptors, GABA-A)
      0 (Zebrafish Proteins)
    • Publication Date:
      Date Created: 20180428 Date Completed: 20180720 Latest Revision: 20190708
    • Publication Date:
      20240104
    • Accession Number:
      PMC5922542
    • Accession Number:
      10.1371/journal.pone.0196083
    • Accession Number:
      29702678