Engineering triterpene metabolism in the oilseed of Arabidopsis thaliana.

Publisher: Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists Country of Publication: England NLM ID: 101201889 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1467-7652 (Electronic) Linking ISSN: 14677644 NLM ISO Abbreviation: Plant Biotechnol J Subsets: MEDLINE

Publication: 2014- : Oxford Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists
Original Publication: [Oxford] : Blackwell Pub., c2003-

Gene Expression Regulation, Plant*; Arabidopsis/*genetics ; Geranyltranstransferase/*metabolism ; Metabolic Engineering/*methods ; Triterpenes/*metabolism; Alkyl and Aryl Transferases/genetics ; Alkyl and Aryl Transferases/metabolism ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Cytosol/metabolism ; Gene Expression Regulation, Enzymologic ; Geranyltranstransferase/genetics ; Mevalonic Acid/metabolism ; Organ Specificity ; Photosynthesis ; Plants, Genetically Modified ; Plastids/metabolism ; Seeds/genetics ; Seeds/metabolism ; Squalene/metabolism

Squalene and botryococcene are linear, hydrocarbon triterpenes that have industrial and medicinal values. While natural sources for these compounds exist, there is a pressing need for robust, renewable production platforms. Oilseeds are an excellent target for heterologous production because of their roles as natural storage repositories and their capacity to produce precursors from photosynthetically-derived carbon. We generated transgenic Arabidopsis thaliana plants using a variety of engineering strategies (subcellular targeting and gene stacking) to assess the potential for oilseeds to produce these two compounds. Constructs used seed-specific promoters and evaluated expression of a triterpene synthase alone and in conjunction with a farnesyl diphosphate synthase (FPS) plus 1-deoxyxylulose 5-phosphate synthase (DXS). Constructs directing biosynthesis to the cytosol to harness isoprenoid precursors from the mevalonic acid (MVA) pathway were compared to those directing biosynthesis to the plastid compartment diverting precursors from the methylerythritol phosphate (MEP) pathway. On average, the highest accumulation for both compounds was achieved by targeting the triterpene synthase, FPS and DXS to the plastid (526.84 μg/g seed for botryococcene and 227.30 μg/g seed for squalene). Interestingly, a higher level accumulation of botryococcene (a non-native compound) was observed when the biosynthetic enzymes were targeted to the cytosol (>1000 μg/g seed in one line), but not squalene (natively produced in the cytosol). Not only do these results indicate the potential of engineering triterpene accumulation in oilseeds, but they also uncover some the unique regulatory mechanisms controlling triterpene metabolism in different cellular compartments of seeds.
(© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)

Plant Physiol. 2014 Jul 1;165(4):1488-1504. (PMID: 24987018)
Nucleic Acids Res. 1989 Jun 12;17(11):4386. (PMID: 2740229)
Nat Prod Rep. 2014 Aug;31(8):1043-55. (PMID: 24921065)
Plant J. 1998 Dec;16(6):735-43. (PMID: 10069079)
Appl Microbiol Biotechnol. 2005 Feb;66(5):486-96. (PMID: 15630516)
Planta. 2001 Mar;212(4):568-72. (PMID: 11525513)
Plant Methods. 2006 Nov 06;2:19. (PMID: 17087829)
Plant Physiol. 2002 May;129(1):321-32. (PMID: 12011362)
Plant Physiol Biochem. 2013 Dec;73:23-32. (PMID: 24036394)
Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):933-8. (PMID: 15630092)
Plant Physiol. 2009 Jul;150(3):1111-21. (PMID: 19403729)
Plant Cell Rep. 2009 Apr;28(4):601-17. (PMID: 19153740)
Plant Physiol. 2002 Sep;130(1):303-11. (PMID: 12226510)
Mol Plant. 2010 Sep;3(5):904-16. (PMID: 20591838)
Planta. 2016 Feb;243(2):549-61. (PMID: 26530959)
Biochimie. 2016 Jan;120:9-16. (PMID: 26107412)
Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12260-5. (PMID: 21746901)
Plant Physiol. 2016 Feb;170(2):702-16. (PMID: 26603654)
Planta. 2012 Sep;236(3):867-77. (PMID: 22729821)
Methods Mol Biol. 1998;82:259-66. (PMID: 9664431)
Phytochemistry. 2014 Feb;98:110-9. (PMID: 24359633)
J Exp Bot. 2009;60(4):1319-32. (PMID: 19204032)
J Biol Chem. 1987 Sep 5;262(25):12196-201. (PMID: 3624251)
Plant J. 1999 Nov;20(4):401-412X. (PMID: 10607293)
Plant Biol (Stuttg). 2005 Jan;7(1):91-7. (PMID: 15666208)
Plant Physiol. 2006 Feb;140(2):466-83. (PMID: 16384899)
Biotechnol Bioeng. 1982 Jan;24(1):193-205. (PMID: 18546110)
Biochemistry. 2014 Dec 9;53(48):7570-81. (PMID: 25393512)
Annu Rev Plant Biol. 2013;64:665-700. (PMID: 23451776)
Planta. 2015 Sep;242(3):693-708. (PMID: 26223979)
Plant J. 1997 May;11(5):1071-8. (PMID: 9193076)
Nat Protoc. 2006;1(2):641-6. (PMID: 17406292)
Nat Biotechnol. 2006 Nov;24(11):1441-7. (PMID: 17057703)
New Phytol. 2012 Mar;193(4):1022-38. (PMID: 22150097)
Phytochemistry. 2006 May;67(9):904-15. (PMID: 16600316)
Prog Lipid Res. 2012 Apr;51(2):95-148. (PMID: 22197147)
Chem Rev. 2011 Oct 12;111(10):6423-51. (PMID: 21902244)
Plant Cell. 1989 Mar;1(3):313-28. (PMID: 2485233)
Biochim Biophys Acta. 2016 Sep;1861(9 Pt A):1049-1057. (PMID: 27320012)
Biotechnol Biofuels. 2015 Oct 29;8:175. (PMID: 26516348)
Biochemistry. 1994 Sep 13;33(36):10871-7. (PMID: 8086404)
J Biol Chem. 2003 Jul 18;278(29):26666-76. (PMID: 12736259)
Development. 1991 Feb;111(2):299-313. (PMID: 1893865)
Plant J. 2003 Oct;36(1):12-20. (PMID: 12974807)
Adv Biochem Eng Biotechnol. 2015;148:161-99. (PMID: 25636485)
Science. 2000 Jan 14;287(5451):303-5. (PMID: 10634784)
J Biol Chem. 2012 Mar 9;287(11):8163-73. (PMID: 22241476)
Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10600-5. (PMID: 9380681)
Nat Biotechnol. 2005 Apr;23(4):482-7. (PMID: 15793573)
Plant J. 2013 Aug;75(3):351-63. (PMID: 23607888)
Nat Biotechnol. 2003 Sep;21(9):1082-7. (PMID: 12897790)

201006141622 International National Institute of Food and Agriculture - USDA

Keywords: isoprenoid; metabolic engineering; oilseed; seed-specific; triterpene

0 (Arabidopsis Proteins)
0 (Triterpenes)
7QWM220FJH (Squalene)
EC 2.5.- (Alkyl and Aryl Transferases)
EC 2.5.1.- (terpene synthase)
EC 2.5.1.10 (Geranyltranstransferase)
S5UOB36OCZ (Mevalonic Acid)

Date Created: 20180707 Date Completed: 20190603 Latest Revision: 20220408

20240105

PMC6335079

10.1111/pbi.12984

29979486