Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS ONE Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
    • Abstract:
      Scalable production of avian cell lines exhibits a valuable potential on therapeutic application by producing recombinant proteins and as the substrate for virus growth due to the special glycosylation occurs in avian species. Chicken primordial germ cells (cPGCs), a germinal pluripotent avian cell type, present the ability of self-renewal, an anchorage-independent cell growth and the ability to be genetically modified. This cell type could be an interesting bioreactor system for industrial purposes. This study sought to establish an expandable culture system with defined components for three-dimensional (3D) culture of cPGCs. cPGCs were cultured in medium supplemented with the functional polymer FP003. Viscoelasticity was low in this medium but cPGCs did not sediment in culture and efficiencies of space and nutrient utilization were thus enhanced and consequently their expansion was improved. The total number of cPGCs increased by 17-fold after 1 week of culture in 3D-FAot medium, an aseric defined medium containing FP003 polymer, FGF2 and Activin A as growth factors and Ovotransferrin as protein. Moreover, cPGC cell lines stably expressed the germline-specific reporter VASA:tdTOMATO, as well as other markers of cPGCs, for more than 1 month upon culture in 3D-FAot medium, indicating that the characteristics of these cells are maintained. In summary, this novel 3D culture system can be used to efficiently expand cPGCs in suspension without mechanical stirring, which is available for long-term culture and no loss of cellular properties was found. This system provides a platform for large-scale culture of cPGCs.
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    • Accession Number:
      0 (Culture Media)
    • Publication Date:
      Date Created: 20180922 Date Completed: 20190211 Latest Revision: 20190215
    • Publication Date:
      20200827
    • Accession Number:
      PMC6150485
    • Accession Number:
      10.1371/journal.pone.0200515
    • Accession Number:
      30240390
  • Citations
    • ABNT:
      CHEN, Y.-C. et al. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PloS one, [s. l.], v. 13, n. 9, p. e0200515, 2018. DOI 10.1371/journal.pone.0200515. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=30240390. Acesso em: 20 out. 2020.
    • AMA:
      Chen Y-C, Chang W-C, Lin S-P, et al. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PloS one. 2018;13(9):e0200515. doi:10.1371/journal.pone.0200515
    • APA:
      Chen, Y.-C., Chang, W.-C., Lin, S.-P., Minami, M., Jean, C., Hayashi, H., Rival-Gervier, S., Kanaki, T., Wu, S.-C., & Pain, B. (2018). Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PloS One, 13(9), e0200515. https://doi.org/10.1371/journal.pone.0200515
    • Chicago/Turabian: Author-Date:
      Chen, Yi-Chen, Wei-Che Chang, Shau-Ping Lin, Masataka Minami, Christian Jean, Hisato Hayashi, Sylvie Rival-Gervier, Tatsuro Kanaki, Shinn-Chih Wu, and Bertrand Pain. 2018. “Three-Dimensional Culture of Chicken Primordial Germ Cells (CPGCs) in Defined Media Containing the Functional Polymer FP003.” PloS One 13 (9): e0200515. doi:10.1371/journal.pone.0200515.
    • Harvard:
      Chen, Y.-C. et al. (2018) ‘Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003’, PloS one, 13(9), p. e0200515. doi: 10.1371/journal.pone.0200515.
    • Harvard: Australian:
      Chen, Y-C, Chang, W-C, Lin, S-P, Minami, M, Jean, C, Hayashi, H, Rival-Gervier, S, Kanaki, T, Wu, S-C & Pain, B 2018, ‘Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003’, PloS one, vol. 13, no. 9, p. e0200515, viewed 20 October 2020, .
    • MLA:
      Chen, Yi-Chen, et al. “Three-Dimensional Culture of Chicken Primordial Germ Cells (CPGCs) in Defined Media Containing the Functional Polymer FP003.” PloS One, vol. 13, no. 9, Sept. 2018, p. e0200515. EBSCOhost, doi:10.1371/journal.pone.0200515.
    • Chicago/Turabian: Humanities:
      Chen, Yi-Chen, Wei-Che Chang, Shau-Ping Lin, Masataka Minami, Christian Jean, Hisato Hayashi, Sylvie Rival-Gervier, Tatsuro Kanaki, Shinn-Chih Wu, and Bertrand Pain. “Three-Dimensional Culture of Chicken Primordial Germ Cells (CPGCs) in Defined Media Containing the Functional Polymer FP003.” PloS One 13, no. 9 (September 21, 2018): e0200515. doi:10.1371/journal.pone.0200515.
    • Vancouver/ICMJE:
      Chen Y-C, Chang W-C, Lin S-P, Minami M, Jean C, Hayashi H, et al. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PloS one [Internet]. 2018 Sep 21 [cited 2020 Oct 20];13(9):e0200515. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=30240390