Predicting tubal factor infertility by using markers of humoral and cell-mediated immune response against Chlamydia trachomatis.

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  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: Denmark NLM ID: 8912860 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0897 (Electronic) Linking ISSN: 10467408 NLM ISO Abbreviation: Am J Reprod Immunol Subsets: MEDLINE
    • Publication Information:
      Publication: Copenhagen : Wiley-Blackwell
      Original Publication: New York : Alan R. Liss, Inc., c1989-
    • Subject Terms:
    • Abstract:
      Problem: The accuracy of Chlamydia trachomatis antibody test in predicting tubal factor infertility (TFI) is limited, and more accurate methods are needed. Cell-mediated immune response (CMI) is crucial in the resolution of pathogen, but it may play an important role in the pathogenesis of C trachomatis-associated tubal damage. We studied whether combining the markers of C trachomatis-induced CMI to humoral immune response improves the accuracy of serology in TFI prediction.
      Method of Study: Our prospective study consists of 258 subfertile women, of whom 22 (8.5%) had TFI. Women with other causes for subfertility served as a reference group. Serum C trachomatis major outer membrane protein (MOMP) and chlamydial heat-shock protein 60 (cHSP60) IgG antibodies were measured by ELISA. CMI was studied by lymphocyte proliferation assay in vitro.
      Results: Serological markers were more prevalent in women with TFI than in other subfertile women (40.9% vs 12.3% for MOMP IgG and 27.3% vs 10.2% for cHSP60 IgG). The best test combination for TFI was C. trachomatis MOMP and cHSP60 antibody with an accuracy of 90.3%, sensitivity of 22.7% and specificity of 96.6%. Positive post-test probability of this combination was 54.2%, and negative post-test probability was 12.4%. Adding of the markers of CMI did not significantly improve the accuracy of serology in TFI prediction.
      Conclusion: The accuracy of TFI prediction increases when the combination of C trachomatis MOMP and cHSP60 antibody tests is used. C trachomatis-induced CMI was common in our study population, but the markers of CMI did not predict TFI.
      (© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
    • Grant Information:
      International Helsingin ja Uudenmaan Sairaanhoitopiiri; International Suomen Akatemia; International Päivikki ja Sakari Sohlbergin Säätiö; International Paulo Foundation
    • Contributed Indexing:
      Keywords: Chlamydia trachomatis; cell-mediated immune response; diagnostic test; serology; tubal evaluation; tubal factor infertility
    • Accession Number:
      0 (Antibodies, Bacterial)
      0 (Biomarkers)
      0 (Chaperonin 60)
      0 (Porins)
      146409-23-6 (omp1 protein, Chlamydia trachomatis)
    • Publication Date:
      Date Created: 20181004 Date Completed: 20191028 Latest Revision: 20191028
    • Publication Date:
      20240105
    • Accession Number:
      10.1111/aji.13051
    • Accession Number:
      30281189