Quantitative analysis of the grain amyloplast proteome reveals differences in metabolism between two wheat cultivars at two stages of grain development.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2000-
    • Subject Terms:
      Plant Development* ; Proteome* ; Proteomics*/methods; Edible Grain/*metabolism ; Plastids/*metabolism ; Triticum/*metabolism; Edible Grain/genetics ; Plastids/genetics ; Protein Interaction Mapping ; Protein Interaction Maps ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Triticum/genetics
    • Abstract:
      Background: Wheat (Triticum aestivum L.) is one of the world's most important grain crops. The amyloplast, a specialized organelle, is the major site for starch synthesis and storage in wheat grain. Understanding the metabolism in amyloplast during grain development in wheat cultivars with different quality traits will provide useful information for potential yield and quality improvement.
      Results: Two wheat cultivars, ZM366 and YM49-198 that differ in kernel hardness and starch characteristics, were used to examine the metabolic changes in amyloplasts at 10 and 15 days after anthesis (DAA) using label-free-based proteome analysis. We identified 523 differentially expressed proteins (DEPs) between 10 DAA and 15 DAA, and 229 DEPs between ZM366 and YM49-198. These DEPs mainly participate in eight biochemical processes: carbohydrate metabolism, nitrogen metabolism, stress/defense, transport, energetics-related, signal transduction, protein synthesis/assembly/degradation, and nucleic acid-related processes. Among these proteins, the DEPs showing higher expression levels at 10 DAA are mainly involved in carbohydrate metabolism, stress/defense, and nucleic acid related processes, whereas DEPs with higher expression levels at 15 DAA are mainly carbohydrate metabolism, energetics-related, and transport-related proteins. Among the DEPs between the two cultivars, ZM366 had more up-regulated proteins than YM49-198, and these are mainly involved in carbohydrate metabolism, nucleic acid-related processes, and transport.
      Conclusions: The results of our study indicate that wheat grain amyloplast has the broad metabolic capability. The DEPs involved in carbohydrate metabolism, nucleic acids, stress/defense, and transport processes, with grain development and cultivar differences, are possibly responsible for different grain characteristics, especially with respect to yield and quality-related traits.
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    • Grant Information:
      2016YFD0300400 the National Key Research and Development Program of China; 162300410137 the Natural Science Foundation of Henan Province
    • Contributed Indexing:
      Keywords: Amyloplast; Grain development; Proteome; Starch characteristics; Wheat
    • Accession Number:
      0 (Proteome)
      0 (RNA, Messenger)
    • Publication Date:
      Date Created: 20181026 Date Completed: 20190211 Latest Revision: 20190215
    • Publication Date:
      20240105
    • Accession Number:
      PMC6201562
    • Accession Number:
      10.1186/s12864-018-5174-z
    • Accession Number:
      30355308