Characterization of T cell receptors in a novel murine model of nickel-induced intraoral metal contact allergy.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Dermatitis, Allergic Contact/*immunology ; Nickel/*immunology ; Receptors, Antigen, T-Cell/*immunology; Animals ; Cytokines/metabolism ; Dermatitis, Allergic Contact/pathology ; Disease Models, Animal ; Female ; Foot ; Gene Expression ; Inflammation/immunology ; Inflammation/pathology ; Lipopolysaccharides ; Lymph Nodes/immunology ; Lymph Nodes/pathology ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Knockout ; Mouth ; RNA, Messenger/metabolism ; T-Lymphocytes/immunology ; T-Lymphocytes/pathology

Nickel is a component of several alloy types that are widely used in our environment, including several dental alloy types that cause intraoral metal contact allergy. However, metal-specific immune responses in the oral mucosa have not been elucidated because a suitable animal model has not been established. In this study, we established a novel murine model of nickel-induced intraoral metal contact allergy and aimed to elucidate the immune response in terms of T-cell receptor repertoire and cytokine profiles in inflamed oral mucosa. The intraoral metal contact allergy model was induced by two sensitizations of nickel plus lipopolysaccharide solution into the postauricular skin followed by a single nickel challenge of the buccal mucosa. Cytokine expression profiles and T-cell phenotypes were determined by quantitative polymerase chain reaction. T cells accumulated in the cervical lymph nodes and inflamed oral mucosa were characterized by analyzing their T-cell receptor α- and β-chain repertoires, and the nucleotide sequences of complementary determining region 3. Significant swelling and pathological features were histologically evident at 1 day after challenge in mice with nickel allergy. At 1 day after the challenge, CD8-positive T cells producing high levels of T helper 1 type cytokines had accumulated in the allergic oral mucosa. At 7 days after the challenge, excessive nickel allergy in the oral mucosa was suppressed by regulatory T cells. Characterization of the T-cell receptor repertoire in nickel allergic mice revealed the presence of natural killer T cells and T cells bearing Trav6-6-Traj57 at 1 day after the challenge. Our murine model of nickel-induced intraoral metal contact allergy showed that natural killer T cells and T cells bearing Trav6-6-Traj57 might be involved in the immune responses of nickel-induced intraoral metal contact allergy.
Competing Interests: The authors have declared that no competing interests exist.

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0 (Cytokines)
0 (Lipopolysaccharides)
0 (RNA, Messenger)
0 (Receptors, Antigen, T-Cell)
7OV03QG267 (Nickel)

Date Created: 20181218 Date Completed: 20190521 Latest Revision: 20200309

20240105

PMC6296741

10.1371/journal.pone.0209248

30557354