A DNA barcode reference library for Swiss butterflies and forester moths as a tool for species identification, systematics and conservation.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
    • Abstract:
      Butterfly monitoring and Red List programs in Switzerland rely on a combination of observations and collection records to document changes in species distributions through time. While most butterflies can be identified using morphology, some taxa remain challenging, making it difficult to accurately map their distributions and develop appropriate conservation measures. In this paper, we explore the use of the DNA barcode (a fragment of the mitochondrial gene COI) as a tool for the identification of Swiss butterflies and forester moths (Rhopalocera and Zygaenidae). We present a national DNA barcode reference library including 868 sequences representing 217 out of 224 resident species, or 96.9% of Swiss fauna. DNA barcodes were diagnostic for nearly 90% of Swiss species. The remaining 10% represent cases of para- and polyphyly likely involving introgression or incomplete lineage sorting among closely related taxa. We demonstrate that integrative taxonomic methods incorporating a combination of morphological and genetic techniques result in a rate of species identification of over 96% in females and over 98% in males, higher than either morphology or DNA barcodes alone. We explore the use of the DNA barcode for exploring boundaries among taxa, understanding the geographical distribution of cryptic diversity and evaluating the status of purportedly endemic taxa. Finally, we discuss how DNA barcodes may be used to improve field practices and ultimately enhance conservation strategies.
      Competing Interests: The authors declare that no competing interests exist. Affiliation with Hintermann & Weber AG does not alter our adherence to PLOS ONE policies on sharing data and materials.
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    • Accession Number:
      0 (Insect Proteins)
      9007-49-2 (DNA)
      EC 1.9.3.1 (Electron Transport Complex IV)
    • Publication Date:
      Date Created: 20181222 Date Completed: 20190507 Latest Revision: 20200309
    • Publication Date:
      20240105
    • Accession Number:
      PMC6303096
    • Accession Number:
      10.1371/journal.pone.0208639
    • Accession Number:
      30576327