Fitness cost of a mcr-1-carrying IncHI2 plasmid.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Escherichia coli/*genetics ; Escherichia coli Proteins/*genetics ; Genome, Bacterial/*genetics ; HSP70 Heat-Shock Proteins/*genetics ; Membrane Proteins/*genetics; Bacterial Outer Membrane Proteins/genetics ; Cell Cycle/genetics ; Cell Division/genetics ; Escherichia coli/growth & development ; Genetic Fitness/genetics ; Plasmids/genetics ; Protein Domains/genetics
    • Abstract:
      IncHI2 is a common type of large mcr-1-carrying plasmids that have been found worldwide. Large plasmids could impose metabolic burden for host bacterial strains, we therefore examine the stability and fitness cost of a mcr-1-carrying 265.5-kb IncHI2 plasmid, pMCR1_1943, in Escherichia coli in nutrient-rich LB and nutrient-restricted M9 broth. Stability tests revealed that pMCR1_1943 was stably maintained with a stability frequency of 0.99±0.01 (mean ± standard deviation) after 880 generations in LB and 0.97±0.00 after 220 generations in M9 broth. Relative fitness (expressed as w, defined as relative fitness of the plasmid-carrying strain compared to the plasmid-free progenitor strain) was examined using the 24-h head to head competitions. pMCR1_1943 initially imposed costs (w, 0.88±0.03 in LB, 0.87±0.01 in M9) but such costs were largely reduced after 14-day cultures (w, 0.97±0.03 in LB, 0.95±0.03 in M9). The stable maintenance and the largely compensated cost after passage may contribute to the wide spread of mcr-1-carrying IncHI2 plasmids. To investigate potential mechanisms for the reduced fitness cost, we performed whole genome sequencing and single nucleotide polymorphism calling for the competitor strains. We identified that molecular chaperone-encoding dnaK, cell division protein-encoding cpoB and repeat protein-encoding rhsC were associated with the cost reduction for pMCR1_1943, which may represent new mechanisms for host bacterial strains to compensate fitness costs imposed by large plasmids and warrant further studies.
      Competing Interests: The authors have declared that no competing interests exist.
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    • Accession Number:
      0 (Bacterial Outer Membrane Proteins)
      0 (Escherichia coli Proteins)
      0 (HSP70 Heat-Shock Proteins)
      0 (MCR-1 protein, E coli)
      0 (Membrane Proteins)
      0 (RhsF protein, E coli)
      0 (YbgF protein, E coli)
      EC 3.6.1.- (dnaK protein, E coli)
    • Publication Date:
      Date Created: 20181227 Date Completed: 20190521 Latest Revision: 20200309
    • Publication Date:
      20240105
    • Accession Number:
      PMC6306219
    • Accession Number:
      10.1371/journal.pone.0209706
    • Accession Number:
      30586457