In vitro osteogenic differentiation of stem cells with different sources on composite scaffold containing natural bioceramic and polycaprolactone.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 101594777 Publication Model: Print Cited Medium: Internet ISSN: 2169-141X (Electronic) Linking ISSN: 21691401 NLM ISO Abbreviation: Artif Cells Nanomed Biotechnol Subsets: MEDLINE
    • Publication Information:
      Publication: 2015- : Abingdon, Oxford : Taylor & Francis
      Original Publication: London : Informa Healthcare, [2013]-
    • Subject Terms:
      Biocompatible Materials/*pharmacology ; Cell Differentiation/*drug effects ; Ceramics/*chemistry ; Osteogenesis/*drug effects ; Polyesters/*chemistry ; Stem Cells/*cytology ; Stem Cells/*drug effects; Biocompatible Materials/chemistry ; Biomarkers/metabolism ; Gene Expression Regulation/drug effects ; Humans ; Nanofibers/chemistry ; Stem Cells/metabolism
    • Abstract:
      Stem cells can be obtained from a variety of sources. To compare the effect of cell source on the osteogenic differentiation potential, buccal fat pad-derived mesenchymal stem cells (BFP-MSCs), bone marrow-derived MSCs (BM-MSCs) and unrestricted somatic stem cells (USSCs) with different accessibility in time and region, were cultured on bioceramic (Bio-Oss ® ) coated electrospun polycaprolactone (PCL) scaffold (PCL-Bio). After scaffold characterization, stem cells proliferation and osteogenic differentiation were investigated by MTT and Alizarin red staining, alkaline phosphatase activity, calcium content and gene expression assays. Proliferation rate of the stem cells was not significantly different with each other, only USSCs showed significantly lower proliferation rate while cultured on PCL-Bio; although, PCL-Bio showed better proliferation support in comparison with tissue culture plate and PCL. Mineralization of the BM-MSCs was significantly higher than others, while BFP-MSCs were close to it. Highest ALP activity was detected in BFP-MSCs cultured on PCL-Bio. USSCs demonstrated higher gene expression level in three genes, although differences were not huge compared to others. According to the results and due to the availability, facilitated preparation procedure and less patients suffering, BFP-MSCs have a better choice than BM-MSCs and USSCs for use in bone tissue engineering.
    • Contributed Indexing:
      Keywords: Buccal fat pad-MSCs; bioceramic; bone marrow-MSCs; polycaprolactone; unrestricted somatic stem cells
    • Accession Number:
      0 (Biocompatible Materials)
      0 (Biomarkers)
      0 (Polyesters)
      24980-41-4 (polycaprolactone)
    • Publication Date:
      Date Created: 20190129 Date Completed: 20190506 Latest Revision: 20190506
    • Publication Date:
      20240104
    • Accession Number:
      10.1080/21691401.2018.1553785
    • Accession Number:
      30688102