Germ cell differentiation of bone marrow mesenchymal stem cells.

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  • Additional Information
    • Source:
      Publisher: Blackwell Pub Country of Publication: Germany NLM ID: 0423506 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0272 (Electronic) Linking ISSN: 03034569 NLM ISO Abbreviation: Andrologia
    • Publication Information:
      Publication: : Berlin : Blackwell Pub.
      Original Publication: Berlin, Grosse
    • Subject Terms:
      Cell Differentiation*; Bone Marrow Cells/*physiology ; Germ Cells/*physiology ; Mesenchymal Stem Cells/*physiology; Animals ; Cell Culture Techniques/methods ; Cell Survival ; Cells, Cultured ; Culture Media, Conditioned ; Flow Cytometry ; Infertility, Male/therapy ; Male ; Mice ; Sertoli Cells ; Tretinoin/metabolism
    • Abstract:
      Bone marrow mesenchymal stem cells (BM-MSCs) were first cultured under induction of retinoic acid (RA), Sertoli cells conditioned medium and RA + con (conditioned medium) as treatment groups. The presence of Sertoli cells was confirmed by immunocytochemistry of follicle-stimulating hormone receptor in Sertoli cells and flow cytometry by anti-Gata4 antibody. Cell viability and morphology of nucleus and cytoplasm of BM-MSCs were evaluated by MTT test and DAPI staining respectively. The expression of Oct4, Plzf, Scp3, Caspases 8, 9 and 3 genes was evaluated by RT-PCR. For increasing the accuracy of experiment, the expression of Vasa and SCP3 genes was investigated quantitatively by real-time PCR after 0, 5, 10, 15 days of culture. The results showed that the number of apoptotic cells increased in RA group. The expression of apoptosis genes (Caspases 3, 8 and 9) was also observed in this group all days of culture. Measurement of Vasa and Scp3 genes by RT-PCR confirmed the positive effects of retinoic acid on increasing of genes expression. So, in this study, a group with maximum expression of differentiation genes and minimum expression of apoptotic genes was RA + conditioned medium group. DNA fragmentation was not observed in all groups.
      (© 2019 Blackwell Verlag GmbH.)
    • Contributed Indexing:
      Keywords: BM-MSCs; apoptosis; differentiation; spermatogenesis
    • Accession Number:
      0 (Culture Media, Conditioned)
      5688UTC01R (Tretinoin)
    • Publication Date:
      Date Created: 20190213 Date Completed: 20190807 Latest Revision: 20190807
    • Publication Date:
      20240105
    • Accession Number:
      10.1111/and.13229
    • Accession Number:
      30746735