Enhanced anti-proliferative and pro-apoptotic effects of metformin encapsulated PLGA-PEG nanoparticles on SKOV3 human ovarian carcinoma cells.

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  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 101594777 Publication Model: Print Cited Medium: Internet ISSN: 2169-141X (Electronic) Linking ISSN: 21691401 NLM ISO Abbreviation: Artif Cells Nanomed Biotechnol Subsets: MEDLINE
    • Publication Information:
      Publication: 2015- : Abingdon, Oxford : Taylor & Francis
      Original Publication: London : Informa Healthcare, [2013]-
    • Subject Terms:
      Apoptosis/*drug effects ; Drug Carriers/*chemistry ; Metformin/*chemistry ; Metformin/*pharmacology ; Nanoparticles/*chemistry ; Ovarian Neoplasms/*pathology ; Polyesters/*chemistry ; Polyethylene Glycols/*chemistry; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Capsules ; Cell Cycle Checkpoints/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Drug Liberation ; Female ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Particle Size
    • Abstract:
      Metformin (MET) has received considerable attention in recent years for its anticancer potential activities. However, short half-life and weak bioavailability of MET limited its use as a chemotherapeutic agent. The present study is intended to evaluate the efficiency of PLGA-PEG as a nano-carrier for MET to increase anticancer effects on SKOV3 ovarian carcinoma cells. MET-loaded PLGA-PEG nanoparticles (NPs) were characterized through Dynamic Light Scattering (DLS), Fourier-transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FE-SEM). Anti-proliferative and apoptotic effects of nanoformulated MET were evaluated using MTT and flow-cytometric assays, respectively. Also, real-time polymerase chain reaction (Real-Time PCR) was used to determine the gene expression levels of apoptotic genes, p53 and hTERT. Evaluation of cytotoxicity showed that MET-NPs had more cytotoxicity than free MET in a time-and dose-dependent manner. The nuclei fragmentation and the percentage of apoptotic cells induced by MET-NPs were significantly higher than free MET. Also, it was found that MET-NPs triggered more cell cycle arrest at sub-G1 checkpoint than free MET. Compared to MET treated cells, the mRNA expression levels of apoptotic genes, as well as p53 and hTERT were significantly altered in MET-NPs treated cells. In conclusion, it is supposed that nano-encapsulation of MET into polymeric PLGA-PEG NPs may be a convenient drug delivery system to enhance its anticancer effects for ovarian cancer therapy.
    • Contributed Indexing:
      Keywords: Metformin; apoptotic effect; ovarian cancer; polymeric nanoparticles
    • Accession Number:
      0 (Antineoplastic Agents)
      0 (Capsules)
      0 (Drug Carriers)
      0 (Polyesters)
      0 (polyethylene glycol-poly(lactide-co-glycolide))
      3WJQ0SDW1A (Polyethylene Glycols)
      9100L32L2N (Metformin)
    • Publication Date:
      Date Created: 20190321 Date Completed: 20190715 Latest Revision: 20190715
    • Publication Date:
      20240105
    • Accession Number:
      10.1080/21691401.2019.1573737
    • Accession Number:
      30892093