Different effects of Wnt/β-catenin activation and parathyroid hormone on diaphyseal and metaphyseal in the early phase of femur bone healing of mice.

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  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: Australia NLM ID: 0425076 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1440-1681 (Electronic) Linking ISSN: 03051870 NLM ISO Abbreviation: Clin Exp Pharmacol Physiol Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford, England : Wiley-Blackwell
      Original Publication: Oxford, Blackwell Scientific Publications.
    • Subject Terms:
    • Abstract:
      Parathyroid hormone (PTH) and agents related to the manipulation of Wnt/β-catenin signalling are two promising anabolic anti-osteoporotic therapies that have been shown to promote the healing of bone fractures. Now, it is widely accepted that cortical bone and trabecular bone are two different compartments, and should be treated as separate compartments in pathological processes, such as fracture healing. It is currently unknown whether PTH and the activation of β-catenin signalling would demonstrate different effects on cortical bone and trabecular bone healing. In the current study, single 0.6-mm cortex holes were made in the femur metaphysis and diaphysis of mice, and then, PTH application and β-catenin activation were used to observe the promoting effect on bone healing. The effects of β-catenin and PTH signalling on fracture healing were observed by X-ray and CT at 3, 6, and 14 days after fracture, and the levels of β-catenin were detected by RT-PCR assay, and the number of specific antigen-positive cells of BRDU, OCN, RUNX2 was counted by immunohistochemical staining. While β-catenin activation and PTH were found to demonstrate similar effects on accelerating metaphyseal bone healing, activation of β-catenin showed a more striking effect than PTH on promoting diaphyseal bone healing. These findings might be helpful for selecting proper medication to accelerate fracture healing of different bone compartments.
      (© 2019 The Authors.Clinical and Experimental Pharmacology and Physiology Published by John Wiley & Sons Australia, Ltd.)
    • References:
      J Orthop Surg Res. 2014 Jun 05;9:45. (PMID: 24902588)
      Bone. 2014 Jun;63:15-9. (PMID: 24582802)
      J Orthop Res. 2017 Apr;35(4):812-819. (PMID: 27306622)
      J Bone Miner Res. 2002 Nov;17(11):2038-47. (PMID: 12412812)
      Curr Osteoporos Rep. 2009 Jul;7(2):64-9. (PMID: 19631031)
      Int J Biol Sci. 2017 Sep 21;13(10):1254-1265. (PMID: 29104492)
      Osteoporos Int. 2011 Feb;22(2):373-90. (PMID: 21085935)
      Calcif Tissue Int. 2012 May;90(5):343-53. (PMID: 22451221)
      Clin Exp Pharmacol Physiol. 2019 Jul;46(7):652-663. (PMID: 30908657)
      Sci Rep. 2017 Jun 2;7(1):2695. (PMID: 28578392)
      Acta Biomater. 2017 Mar 15;51:513-525. (PMID: 28088671)
      N Engl J Med. 2008 Mar 20;358(12):1304-6. (PMID: 18354114)
      Int J Biol Sci. 2016 Jul 17;12(8):990-9. (PMID: 27489502)
      Clin Orthop Relat Res. 2001 Nov;(392):427-32. (PMID: 11716418)
      J Bone Miner Res. 2013 Sep;28(9):1898-911. (PMID: 23716486)
      J Clin Endocrinol Metab. 2015 Jul;100(7):2718-23. (PMID: 25933031)
      Lancet. 2010 May 15;375(9727):1729-36. (PMID: 20472174)
      Bone. 2014 Feb;59:173-9. (PMID: 24275677)
      PLoS One. 2013 Nov 08;8(11):e81399. (PMID: 24260576)
      J Orthop Trauma. 2011 Jun;25 Suppl 2:S51-5. (PMID: 21566475)
      J Bone Miner Res. 2007 Dec;22(12):1913-23. (PMID: 17696762)
      Calcif Tissue Int. 2015 Dec;97(6):581-91. (PMID: 26314884)
      Bone. 2015 Dec;81:614-619. (PMID: 26232373)
      J Bone Miner Res. 2010 Feb;25(2):404-14. (PMID: 19594305)
      Folia Morphol (Warsz). 2019;78(3):588-594. (PMID: 30644082)
      Clin Exp Pharmacol Physiol. 2013 Nov;40(11):715-23. (PMID: 23937065)
      J Bone Miner Res. 2010 Nov;25(11):2412-8. (PMID: 20499342)
      Bone. 2015 Dec;81:104-111. (PMID: 26164475)
      World J Orthop. 2014 Jul 18;5(3):247-54. (PMID: 25035827)
      Dev Biol. 2002 Apr 15;244(2):305-18. (PMID: 11944939)
      Clin Orthop Relat Res. 2004 Aug;(425):12-25. (PMID: 15292783)
      Bone Rep. 2017 Nov 24;8:1-8. (PMID: 29276733)
      J Bone Joint Surg Br. 2001 Apr;83(3):437-40. (PMID: 11341434)
      J Bone Miner Res. 2011 Jul;26(7):1577-83. (PMID: 21312271)
      Biochem Biophys Res Commun. 2013 Nov 29;441(4):886-90. (PMID: 24211207)
      J Bone Miner Res. 2002 Dec;17(12):2237-46. (PMID: 12469918)
      Int J Biol Sci. 2017 Jul 18;13(8):996-1007. (PMID: 28924381)
      J Gerontol A Biol Sci Med Sci. 2013 Oct;68(10):1218-25. (PMID: 23833200)
      Exp Cell Res. 2017 Jan 1;350(1):123-131. (PMID: 27865936)
      Acta Orthop. 2018 Aug;89(4):457-461. (PMID: 29865916)
      J Bone Miner Res. 2015 Jul;30(7):1184-94. (PMID: 25639729)
      Zhonghua Yi Xue Za Zhi. 2018 Mar 13;98(10):781-787. (PMID: 29562406)
      Chem Biol Interact. 2019 Feb 25;300:101-110. (PMID: 30639440)
      PLoS Med. 2007 Jul 31;4(7):e249. (PMID: 17676991)
      Bone. 2015 Feb;71:63-75. (PMID: 25263522)
      Clin Orthop Relat Res. 2008 May;466(5):1047-53. (PMID: 18288545)
      J Orthop Res. 2014 Mar;32(3):471-6. (PMID: 24243768)
      Clin Orthop Relat Res. 2011 Aug;469(8):2215-24. (PMID: 21132409)
      Clin Cases Miner Bone Metab. 2013 May;10(2):116-20. (PMID: 24133528)
      Bone. 2011 May 1;48(5):988-96. (PMID: 21329773)
      J Bone Miner Res. 2010 Aug;25(8):1886-94. (PMID: 20222106)
      Acta Orthop. 2011 Aug;82(4):465-70. (PMID: 21689069)
      Clin Orthop Relat Res. 2016 May;474(5):1294-302. (PMID: 26608966)
      Ann Anat. 2017 Mar;210:76-83. (PMID: 27986617)
    • Contributed Indexing:
      Keywords: Wnt/β-catenin; bone healing; diaphysis; metaphysis; parathyroid hormone
    • Accession Number:
      0 (Parathyroid Hormone)
      0 (Wnt Proteins)
      0 (beta Catenin)
    • Publication Date:
      Date Created: 20190326 Date Completed: 20200706 Latest Revision: 20210109
    • Publication Date:
      20240105
    • Accession Number:
      PMC6593981
    • Accession Number:
      10.1111/1440-1681.13088
    • Accession Number:
      30908657