Ehrlichia species in pond-farmed leeches (Hirudinaria sp.) in Hubei Province, China.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Ehrlichia/*isolation & purification ; Ehrlichiosis/*veterinary ; Electron Transport Complex IV/*genetics ; Leeches/*microbiology; Animals ; China/epidemiology ; DNA, Bacterial/genetics ; Ehrlichia/genetics ; Ehrlichiosis/epidemiology ; Ehrlichiosis/microbiology ; Farms ; Humans ; Mitochondria/enzymology ; RNA, Ribosomal, 18S/genetics
    • Abstract:
      Leeches are frequently used in traditional Chinese medicine. However, they are potentially hazardous to human and animal health by transmitting several pathogens. Studies of diseases transmitted by leeches are scarce. The purpose of this study was to analyze the pathogens carried in pond-farmed medicinal leech in China. Leeches were collected from 6 farms in Hubei Province in central China. DNA was extracted from the internal organ of leeches to analyze the origin of blood meal. Leech genera were confirmed through amplification of 18S rRNA and mitochondrial gene cytochrome oxidase I (COI) gene by PCR and host animal species were identified through amplification of mitochondrial cytochrome b gene. Species of Ehrlichia in the leech specimens were screened with PCR using specific primers. PCR amplification and DNA sequencing showed that 620 leeches were Hirudinaria sp. Ehrlichia DNA was detected in 39 specimens from 2 farms. We obtained a total of 65 sequences of the cytB gene from 620 leech internal organ samples including sequences of human (n = 5), rat (n = 1), domestic pig (n = 10), duck (n = 23), goose (n = 12) and buffalo (n = 14). Phylogenetic analysis of the rrs and groEL gene sequences showed that Ehrlichia detected in the study were closely related to Ehrlichia sp. in ticks from Korea and Japan. To the best of our knowledge, this is the first report on Ehrlichia DNA being detected from leeches. Our findings provided new data on Ehrlichia spp. and farmed leech species in China.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      Ticks Tick Borne Dis. 2017 Jun;8(4):574-580. (PMID: 28411028)
      J Clin Microbiol. 1997 Jul;35(7):1681-6. (PMID: 9196173)
      Appl Environ Microbiol. 2006 Feb;72(2):1102-9. (PMID: 16461655)
      Parasit Vectors. 2016 Jun 07;9(1):326. (PMID: 27267358)
      Clin Infect Dis. 2007 Jul 15;45 Suppl 1:S45-51. (PMID: 17582569)
      J Exp Med. 1957 Apr 1;105(4):373-82. (PMID: 13416474)
      Vector Borne Zoonotic Dis. 2005 Summer;5(2):162-71. (PMID: 16011433)
      Microb Pathog. 2017 Sep;110:275-278. (PMID: 28705746)
      N Engl J Med. 1999 Jul 15;341(3):148-55. (PMID: 10403852)
      Clin Microbiol Rev. 2003 Jan;16(1):37-64. (PMID: 12525424)
      J Med Microbiol. 2011 Jun;60(Pt 6):787-92. (PMID: 21372183)
      Nat Methods. 2012 Jul 30;9(8):772. (PMID: 22847109)
      Mol Phylogenet Evol. 1999 Aug;12(3):350-9. (PMID: 10413628)
      Parasitol Res. 1994;80(4):277-90. (PMID: 8073013)
      Vet Parasitol. 2010 Feb 10;167(2-4):123-35. (PMID: 19836892)
      Vector Borne Zoonotic Dis. 2016 Jun;16(6):363-7. (PMID: 27135624)
      Proc Natl Acad Sci U S A. 2015 Dec 1;112(48):E6593-4. (PMID: 26598652)
      Ann Intern Med. 1988 Sep 1;109(5):399-405. (PMID: 3044211)
      Iran J Vet Res. 2015 Spring;16(2):218-22. (PMID: 27175180)
      Clin Lab Med. 2010 Mar;30(1):261-92. (PMID: 20513551)
      Sci Rep. 2016 Dec 09;6:38770. (PMID: 27934910)
      Ann Agric Environ Med. 2003;10(2):137-41. (PMID: 14677903)
      Microb Ecol. 2005 Feb;49(2):265-71. (PMID: 15965725)
      Complement Ther Clin Pract. 2010 Nov;16(4):213-5. (PMID: 20920805)
      Korean J Parasitol. 2014 Apr;52(2):169-75. (PMID: 24850960)
      Virology. 2004 Mar 30;321(1):101-10. (PMID: 15033569)
      Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9. (PMID: 7881515)
      Vet Sci. 2016 Aug 31;3(3):. (PMID: 29056728)
      Mol Biol Evol. 2016 Jul;33(7):1870-4. (PMID: 27004904)
      Parasit Vectors. 2015 Mar 23;8:144. (PMID: 25889750)
      Appl Environ Microbiol. 2008 Apr;74(8):2461-70. (PMID: 18296538)
    • Accession Number:
      0 (DNA, Bacterial)
      0 (RNA, Ribosomal, 18S)
      EC 1.9.3.1 (Electron Transport Complex IV)
    • Publication Date:
      Date Created: 20190409 Date Completed: 20200106 Latest Revision: 20200309
    • Publication Date:
      20240105
    • Accession Number:
      PMC6453479
    • Accession Number:
      10.1371/journal.pone.0215082
    • Accession Number:
      30958857