The Solanum chacoense Fertilization-Related Kinase 3 (ScFRK3) is involved in male and female gametophyte development.

Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Ovule/*growth & development ; Plant Proteins/*metabolism ; Pollen/*growth & development ; Protein Kinases/*metabolism ; Solanum/*growth & development; In Situ Hybridization ; Phylogeny ; Plant Proteins/genetics ; Plant Proteins/physiology ; Protein Kinases/genetics ; Protein Kinases/physiology ; RNA, Plant/metabolism ; Solanum/enzymology ; Solanum/genetics ; Two-Hybrid System Techniques

Background: The Fertilization-related kinases (FRK) form a class that belongs to the MEKK subfamily of plant MAPKKKs. It was recently shown that FRK class kinases expanded during angiosperm evolution, reaching their maximum numbers in the lineage leading to solanaceous species and culminating in the Solanum genus where they account for more than 40% of the total MEKKs. The first members studied, ScFRK1 and ScFRK2 were shown to play a pivotal role in gametophyte development in the wild potato species Solanum chacoense.
Results: ScFRK3 is also involved in gametophyte development. ScFRK3 is expressed in developing pollen and young ovules, reaching its highest level immediately after meiosis and during the mitosis steps in both gametophytes. Hence, three independent lines of ScFRK3 RNAi mutant plants showed decreased number of seeds per fruit. We also observed an important number of degenerated embryo sac in mature ovary. Analysis of ovule development showed that most embryo sac did not enter mitosis I in ScFRK3 RNAi mutant plants. Severe lethality was also observed during male gametophyte development, pollen being arrested before mitosis I, as observed in the female gametophyte. Obvious defects in vegetative organs were not observed, emphasizing the reproductive roles of the FRK class kinases. To isolate MAP kinases acting downstream of ScFRK3, a de novo S. chacoense transcriptome from male and female reproductive organs was assembled. Of the five ScMKKs and 16 ScMPKs retrieved, only the ScMKK3 interacted with ScFRK3, while only the ScMPK13 interacted with ScMKK3, leading to an apparent single three-tiered canonical MAP kinase cascade combination involving ScFRK3-ScMKK3-ScMPK13.
Conclusions: The ScFRK3 MAPKKK is involved in a signaling cascade that regulates both male and female gamete development, and most probably act upstream of ScMKK3 and ScMPK13.

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RGPIN-2014-03883 Natural Sciences and Engineering Research Council of Canada

Keywords: Embryo sac; Gametophyte; Mitogen-activated protein kinase (MAPK or MPK); Mitogen-activated protein kinase kinase (MAPKK or MEK); Mitogen-activated protein kinase kinase kinase (MAPKKK or MEKK); Pollen; Reproduction; Signaling cascade; Solanum chacoense

0 (Plant Proteins)
0 (RNA, Plant)
EC 2.7.- (Protein Kinases)

Date Created: 20190518 Date Completed: 20190625 Latest Revision: 20200225

20240104

PMC6524262

10.1186/s12870-019-1804-0

31096905