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Alternative polyadenylation of single cells delineates cell types and serves as a prognostic marker in early stage breast cancer.
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- Author(s): Kim N;Kim N;Kim N; Chung W; Chung W; Eum HH; Eum HH; Eum HH; Lee HO; Lee HO; Lee HO; Lee HO; Park WY; Park WY; Park WY; Park WY; Park WY
- Source:
PloS one [PLoS One] 2019 May 17; Vol. 14 (5), pp. e0217196. Date of Electronic Publication: 2019 May 17 (Print Publication: 2019).- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
- Publication Information: Original Publication: San Francisco, CA : Public Library of Science
- Subject Terms: Gene Expression Regulation, Neoplastic* ; Polyadenylation*; 3' Untranslated Regions/*genetics ; Breast Neoplasms/*diagnosis ; Kidney Neoplasms/*diagnosis ; MicroRNAs/*genetics ; RNA, Messenger/*metabolism ; Single-Cell Analysis/*methods; Biomarkers, Tumor/analysis ; Breast Neoplasms/genetics ; Cell Proliferation ; Female ; Gene Expression Profiling ; Humans ; Kidney Neoplasms/genetics ; Prognosis ; RNA, Messenger/genetics ; Sequence Analysis, RNA ; Tumor Microenvironment
- Abstract: Alternative polyadenylation (APA) in 3' untranslated regions (3' UTR) plays an important role in regulating transcript abundance, localization, and interaction with microRNAs. Length-variation of 3'UTRs by APA contributes to efficient proliferation of cancer cells. In this study, we investigated APA in single cancer cells and tumor microenvironment cells to understand the physiological implication of APA in different cell types. We analyzed APA patterns and the expression level of genes from the 515 single-cell RNA sequencing (scRNA-seq) dataset from 11 breast cancer patients. Although the overall 3'UTR length of individual genes was distributed equally in tumor and non-tumor cells, we found a differential pattern of polyadenylation in gene sets between tumor and non-tumor cells. In addition, we found a differential pattern of APA across tumor types using scRNA-seq data from 3 glioblastoma patients and 1 renal cell carcinoma patients. In detail, 1,176 gene sets and 53 genes showed the distinct pattern of 3'UTR shortening and over-expression as signatures for five cell types including B lymphocytes, T lymphocytes, myeloid cells, stromal cells, and breast cancer cells. Functional categories of gene sets for cellular proliferation demonstrated concordant regulation of APA and gene expression specific to cell types. The expression of APA genes in breast cancer was significantly correlated with the clinical outcome of earlier stage breast cancer patients. We identified cell type-specific APA in single cells, which allows the identification of cell types based on 3'UTR length variation in combination with gene expression. Specifically, an immune-specific APA signature in breast cancer could be utilized as a prognostic marker of early stage breast cancer.
Competing Interests: W.P. is employed by a commercial company, GENINUS Inc. This does not alter our adherence to PLOS ONE policies on sharing data and materials. - References: Neoplasia. 2017 Jul;19(7):574-582. (PMID: 28624626)
Nat Commun. 2014 Nov 20;5:5274. (PMID: 25409906)
Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9157-62. (PMID: 24927563)
Nat Rev Mol Cell Biol. 2017 Jan;18(1):18-30. (PMID: 27677860)
BMC Bioinformatics. 2016 Oct 18;17(1):423. (PMID: 27756200)
Mol Syst Biol. 2015 Jun 03;11(6):812. (PMID: 26040288)
Results Probl Cell Differ. 2017;62:365-376. (PMID: 28455717)
Nature. 2008 Nov 27;456(7221):470-6. (PMID: 18978772)
Science. 2014 Jun 20;344(6190):1396-401. (PMID: 24925914)
Immunity. 1999 Mar;10(3):323-32. (PMID: 10204488)
Genome Res. 2011 May;21(5):741-7. (PMID: 21474764)
Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886)
Cell Tissue Res. 2013 Dec;354(3):783-92. (PMID: 24036843)
Curr Protoc Bioinformatics. 2014 Sep 08;47:11.12.1-34. (PMID: 25199790)
Science. 2016 Apr 8;352(6282):189-96. (PMID: 27124452)
Oncotarget. 2016 Sep 13;7(37):59834-59844. (PMID: 27494850)
Mol Cell. 2011 Sep 16;43(6):853-66. (PMID: 21925375)
Transpl Immunol. 2006 Dec;17(1):23-6. (PMID: 17157209)
J Immunol. 2017 May 1;198(9):3375-3379. (PMID: 28416714)
Nat Med. 2015 May;21(5):467-475. (PMID: 25894827)
Mol Immunol. 2011 Mar;48(6-7):941-6. (PMID: 21251717)
Int J Oncol. 2000 Feb;16(2):295-303. (PMID: 10639573)
Nat Methods. 2010 Dec;7(12):1009-15. (PMID: 21057496)
Mol Cancer Res. 2017 Dec;15(12):1741-1751. (PMID: 28912168)
Genome Res. 2003 Nov;13(11):2498-504. (PMID: 14597658)
Front Immunol. 2018 May 31;9:1235. (PMID: 29904389)
BMC Bioinformatics. 2011 Aug 04;12:323. (PMID: 21816040)
Nat Commun. 2017 May 05;8:15081. (PMID: 28474673)
Bioinformatics. 2015 Jun 1;31(11):1845-7. (PMID: 25617413)
Nat Commun. 2017 Sep 6;8(1):451. (PMID: 28878208)
Cell. 2009 Aug 21;138(4):673-84. (PMID: 19703394)
Brain Pathol. 2016 Jan;26(1):3-17. (PMID: 26269128)
J Cancer Res Clin Oncol. 2013 Dec;139(12):2125-32. (PMID: 24146193)
Nat Genet. 2017 Apr;49(4):594-599. (PMID: 28263318)
Hum Mol Genet. 2015 Dec 15;24(24):6910-20. (PMID: 26395459)
Nucleic Acids Res. 2007 Jan;35(Database issue):D165-8. (PMID: 17202160)
Genome Biol. 2016 Apr 29;17:80. (PMID: 27139883)
Bioinformatics. 2014 Aug 1;30(15):2162-70. (PMID: 24728858)
Biochem Biophys Res Commun. 2014 Oct 10;453(1):7-12. (PMID: 25234598)
Science. 2008 Jun 20;320(5883):1643-7. (PMID: 18566288) - Accession Number: 0 (3' Untranslated Regions)
0 (Biomarkers, Tumor)
0 (MicroRNAs)
0 (RNA, Messenger) - Publication Date: Date Created: 20190518 Date Completed: 20200129 Latest Revision: 20200309
- Publication Date: 20240104
- Accession Number: PMC6524824
- Accession Number: 10.1371/journal.pone.0217196
- Accession Number: 31100099
- Source:
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