The acquisition of long-lived memory B cell responses to merozoite surface protein-8 in individuals with Plasmodium vivax infection.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101139802 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2875 (Electronic) Linking ISSN: 14752875 NLM ISO Abbreviation: Malar J
    • Publication Information:
      Original Publication: London : BioMed Central, [2002-
    • Subject Terms:
    • Abstract:
      Background: The ability of a malaria antigen to induce effective, long-lasting immune responses is important for the development of a protective malaria vaccine. Plasmodium vivax merozoite surface protein-8 (PvMSP8) has been shown to be immunogenic in natural P. vivax infections and produces both cell-mediated and antibody-mediated immunity. Thus, PvMSP8 has been proposed as a vaccine candidate following fusion with other merozoite antigens in blood stage vaccine design. Here, the long-term responses of antibodies and memory B cells (MBCs) specific to PvMSP8 in individuals were monitored in a longitudinal cohort study.
      Methods: Both cross-sectional surveys and cohort studies were utilized to explore the persistence of antibody and MBC responses to PvMSP8. Antibody titers were detected in individuals with acute disease and those who recovered from an infection for 4 years. The dominant peptide epitope of PvMSP8 recognized by naturally acquired antibodies was examined to observe the durability of the post-infection antibody response. PvMSP8-specific MBCs were also in subjects 4 years post-infection using an enzyme-linked immunospot assay.
      Results: The prevalence of antibodies to PvMSP8 was high during and after infection. The antibody levels in individual responders were monitored for up to 12 months post-infection and showed that most patients maintained their seropositive response. Interestingly, the anti-PvMSP8 antibody responses stably persisted in some patients who had recovered from an infection for 4 years. Positive PvMSP8-specific MBCs were also detected at 4 years post-infection. However, analysis in these individuals showed no correlation with the presence or titer of circulating antibody.
      Conclusion: PvMSP8 had the ability to induce a long-term humoral immune response. The antibodies and MBCs specific for this antigen developed and persisted in subjects who acquired a natural P. vivax infection. Inclusion of the PvMSP8 antigen in blood stage vaccine design should be considered.
    • References:
      Mol Biochem Parasitol. 2001 May;114(2):217-26. (PMID: 11378201)
      Immunol Cell Biol. 2002 Oct;80(5):401-14. (PMID: 12225376)
      Infect Immun. 2005 Jul;73(7):3912-22. (PMID: 15972477)
      Trends Parasitol. 2008 Dec;24(12):578-84. (PMID: 18848497)
      J Immunol. 2009 Aug 1;183(3):2176-82. (PMID: 19592645)
      Infect Immun. 2010 Mar;78(3):1089-95. (PMID: 20008533)
      PLoS Pathog. 2010 Feb 19;6(2):e1000770. (PMID: 20174609)
      Vaccine. 2010 Oct 4;28(42):6876-84. (PMID: 20709001)
      Infect Immun. 2010 Nov;78(11):4613-24. (PMID: 20823210)
      Infect Immun. 2012 Apr;80(4):1583-92. (PMID: 22252876)
      Infect Immun. 2012 Jul;80(7):2473-84. (PMID: 22585960)
      Infect Immun. 2013 Oct;81(10):3843-54. (PMID: 23897613)
      Front Immunol. 2014 Jul 28;5:359. (PMID: 25120545)
      Elife. 2015 May 08;4:null. (PMID: 25955968)
      Malar J. 2016 Mar 15;15:159. (PMID: 26975721)
      Malar J. 2017 May 22;16(1):211. (PMID: 28532483)
      JCI Insight. 2017 Jun 15;2(12):null. (PMID: 28614791)
      Malar J. 2017 Aug 30;16(1):354. (PMID: 28854974)
      Infect Immun. 2017 Dec 19;86(1):. (PMID: 28993460)
      Sci Rep. 2018 May 29;8(1):8347. (PMID: 29844379)
    • Grant Information:
      RSA6280064 Thailand Research Funds; PHD/0162/2560 Royal Golden Jubilee PhD Scholarship; NRF-2017R1A2A2A05069562 National Research Foundation of Korea; NRF-2015R1A4A1038666 National Research Foundation of Korea
    • Contributed Indexing:
      Keywords: Memory B cells; Merozoite surface protein 8; Plasmodium vivax
    • Accession Number:
      0 (Antibodies, Protozoan)
      0 (Antigens, Protozoan)
      0 (Immunoglobulin G)
      0 (Protozoan Proteins)
      0 (merozoite surface protein 8, Plasmodium)
    • Publication Date:
      Date Created: 20190602 Date Completed: 20190812 Latest Revision: 20200225
    • Publication Date:
      20240105
    • Accession Number:
      PMC6545020
    • Accession Number:
      10.1186/s12936-019-2821-z
    • Accession Number:
      31151441