Extensive genome analysis of Coxiella burnetii reveals limited evolution within genomic groups.

Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics

Original Publication: London : BioMed Central, [2000-

Genome, Bacterial*; Coxiella burnetii/*genetics; Animals ; Cattle ; Coxiella burnetii/classification ; Coxiella burnetii/isolation & purification ; Evolution, Molecular ; Genome-Wide Association Study ; Genomics ; Genotyping Techniques ; Phylogeny ; United Kingdom

Background: Coxiella burnetii is a zoonotic pathogen that resides in wild and domesticated animals across the globe and causes a febrile illness, Q fever, in humans. An improved understanding of the genetic diversity of C. burnetii is essential for the development of diagnostics, vaccines and therapeutics, but genotyping data is lacking from many parts of the world. Sporadic outbreaks of Q fever have occurred in the United Kingdom, but the local genetic make-up of C. burnetii has not been studied in detail.
Results: Here, we report whole genome data for nine C. burnetii sequences obtained in the UK. All four genomes of C. burnetii from cattle, as well as one sheep sample, belonged to Multi-spacer sequence type (MST) 20, whereas the goat samples were MST33 (three genomes) and MST32 (one genome), two genotypes that have not been described to be present in the UK to date. We established the phylogenetic relationship between the UK genomes and 67 publically available genomes based on single nucleotide polymorphisms (SNPs) in the core genome, which confirmed tight clustering of strains within genomic groups, but also indicated that sub-groups exist within those groups. Variation is mainly achieved through SNPs, many of which are non-synonymous, thereby confirming that evolution of C. burnetii is based on modification of existing genes. Finally, we discovered genomic-group specific genome content, which supports a model of clonal expansion of previously established genotypes, with large scale dissemination of some of these genotypes across continents being observed.
Conclusions: The genetic make-up of C. burnetii in the UK is similar to the one in neighboring European countries. As a species, C. burnetii has been considered a clonal pathogen with low genetic diversity at the nucleotide level. Here, we present evidence for significant variation at the protein level between isolates of different genomic groups, which mainly affects secreted and membrane-associated proteins. Our results thereby increase our understanding of the global genetic diversity of C. burnetii and provide new insights into the evolution of this emerging zoonotic pathogen.

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United Kingdom WT_ Wellcome Trust; MR/M008924/1 United Kingdom MRC_ Medical Research Council

Keywords: Coxiella burnetii; Genotyping; Pan-Genome Analysis; Patho-adaptation; Whole Genome Sequencing

Date Created: 20190606 Date Completed: 20190826 Latest Revision: 20240214

20240214

PMC6549354

10.1186/s12864-019-5833-8

31164106