Genome-wide analysis of DNA methylation identifies novel differentially methylated regions associated with lipid accumulation improved by ethanol extracts of Allium tubersosum and Capsella bursa-pastoris in a cell model.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Genome-Wide Association Study* ; Models, Biological*; Allium/*chemistry ; Capsella/*chemistry ; DNA Methylation/*genetics ; Ethanol/*chemistry ; Lipid Metabolism/*genetics ; Plant Extracts/*pharmacology; DNA Methylation/drug effects ; Fatty Acid Synthases/metabolism ; Fatty Liver/drug therapy ; Gene Expression Regulation/drug effects ; Genome, Human ; Hep G2 Cells ; Humans ; Lipid Metabolism/drug effects ; Plant Extracts/therapeutic use ; Sequence Analysis, DNA
    • Abstract:
      Hepatic steatosis is the most common chronic liver disease in Western countries. Both genetic and environmental factors are known as causes of the disease although their underlying mechanisms have not been fully understood. This study investigated the association of DNA methylation with oleic acid-induced hepatic steatosis. It also examined effects of food components on DNA methylation in hepatic steatosis. Genome-wide DNA methylation of oleic acid (OA)-induced lipid accumulation in vitro cell model was investigated using reduced representation bisulfite sequencing. Changes of DNA methylation were also analyzed after treatment with food components decreasing OA-induced lipid accumulation in the model. We identified total 81 regions that were hypermethylated by OA but hypomethylated by food components or vice versa. We determined the expression of seven genes proximally located at the selected differentially methylated regions. Expression levels of WDR27, GNAS, DOK7, MCF2L, PRKG1, and CMYA5 were significantly different between control vs OA and OA vs treatment with food components. We demonstrated that DNA methylation was associated with expression of genes in the model of hepatic steatosis. We also found that food components reversely changed DNA methylation induced by OA and alleviated lipid accumulation. These results suggest that DNA methylation is one of the mechanisms causing the hepatic steatosis and its regulation by food components provides insights that may prevent or alleviate lipid accumulation.
      Competing Interests: The authors declare that they have no conflict of interest.
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    • Accession Number:
      0 (Plant Extracts)
      3K9958V90M (Ethanol)
      EC 2.3.1.85 (Fatty Acid Synthases)
    • Publication Date:
      Date Created: 20190607 Date Completed: 20200211 Latest Revision: 20200309
    • Publication Date:
      20240105
    • Accession Number:
      PMC6553759
    • Accession Number:
      10.1371/journal.pone.0217877
    • Accession Number:
      31170227