Piezo1 induced apoptosis of type II pneumocytes during ARDS.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Source:
      Publisher: BioMed Central Ltd Country of Publication: England NLM ID: 101090633 Publication Model: Electronic Cited Medium: Internet ISSN: 1465-993X (Electronic) Linking ISSN: 14659921 NLM ISO Abbreviation: Respir Res Subsets: MEDLINE
    • Publication Information:
      Publication: 2001- : London : BioMed Central Ltd.
      Original Publication: London : Current Science Ltd., c2000-
    • Subject Terms:
    • Abstract:
      Objective: The mechanisms of lung injury in acute respiratory distress syndrome (ARDS) are not well understood.Piezo1 was recently identified as a mechanotransduction protein. The present study found the expression of Piezo1 in type II pneumocytes and investigated its role in mediating ARDS-related lung injury.
      Methods: Sprague-Dawley rats were used to establish an ARDS model, the expression of Piezo1,lung injuries, apoptosis as well as calcium influx were assessed.
      Results: Piezo1 was expressed in type II pneumocytes as shown by immunofluorescence staining and expression was increased in the ARDS model. Knockdown of Piezo1 reduced apoptosis which was related to the elevation of Bcl-2.Calcium influx played a vital role in Piezo1-induced apoptosis.
      Conclusion: Piezo1 was expressed in type II pneumocytes. Mechanical stretch of alveoli during ARDS induced activation of the Piezo1 channel,which resulted in calcium influx. The increased intracellular Ca2+ induced the apoptosis of type II pneumocytes, which may be related to the Bcl-2 pathway.
    • References:
      Cold Spring Harb Perspect Biol. 2013 Feb 01;5(2):. (PMID: 23378584)
      EMBO Rep. 2013 Dec;14(12):1143-8. (PMID: 24157948)
      Biochem Biophys Res Commun. 2019 May 21;513(1):147-153. (PMID: 30948157)
      Life Sci. 2016 Dec 1;166:1-7. (PMID: 27756599)
      Cell Tissue Res. 2017 Mar;367(3):495-509. (PMID: 27796509)
      Am J Respir Crit Care Med. 2010 Mar 15;181(6):578-86. (PMID: 19910610)
      Exp Lung Res. 2017 Feb;43(1):49-56. (PMID: 28394655)
      Thromb Haemost. 2016 Aug 1;116(2):272-84. (PMID: 27277069)
      J Appl Physiol (1985). 2004 Oct;97(4):1535-42. (PMID: 15358756)
      Elife. 2015 May 22;4:. (PMID: 26001274)
      J Biol Chem. 2017 Jun 2;292(22):9204-9217. (PMID: 28416610)
      J Clin Invest. 2016 Dec 1;126(12):4527-4536. (PMID: 27797339)
      J Biol Chem. 2014 Nov 14;289(46):31673-31681. (PMID: 25305018)
      Pflugers Arch. 2016 Jul;468(7):1197-1206. (PMID: 27023350)
      J Intensive Care. 2014 Feb 18;2(1):10. (PMID: 25520826)
      JAMA. 2016 Feb 23;315(8):788-800. (PMID: 26903337)
      Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10347-52. (PMID: 24958852)
      Nature. 2014 Nov 13;515(7526):279-282. (PMID: 25119035)
      Zhonghua Jie He He Hu Xi Za Zhi. 1999 Oct;22(10):610-2. (PMID: 11776551)
      Cell Mol Life Sci. 2019 Oct;76(19):3843-3859. (PMID: 30989245)
      Mol Cell. 2008 Jul 25;31(2):255-65. (PMID: 18657507)
      Annu Rev Pathol. 2011;6:147-63. (PMID: 20936936)
      Nat Rev Nephrol. 2017 Jan;13(1):3. (PMID: 27840417)
      Cell Rep. 2016 Nov 8;17(7):1739-1746. (PMID: 27829145)
      Am J Respir Crit Care Med. 2008 Aug 15;178(4):346-55. (PMID: 18451319)
      Exp Lung Res. 1982 May;3(2):163-73. (PMID: 6286299)
      Cell Rep. 2015 Nov 10;13(6):1161-1171. (PMID: 26526998)
      Science. 2010 Oct 1;330(6000):55-60. (PMID: 20813920)
      Cell Immunol. 1988 Jan;111(1):139-47. (PMID: 3123069)
      J Biol Chem. 2014 Jun 6;289(23):16565-75. (PMID: 24759099)
      Pathol Res Pract. 2010 Mar 15;206(3):145-50. (PMID: 20097014)
      Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11783-8. (PMID: 26351678)
      Haematologica. 2014 Jan;99(1):70-5. (PMID: 23872304)
      Biochem Biophys Res Commun. 2014 Oct 10;453(1):101-5. (PMID: 25256743)
      JAMA. 2012 Jun 20;307(23):2526-33. (PMID: 22797452)
      In Vitro Cell Dev Biol Anim. 1995 May;31(5):361-6. (PMID: 7543342)
      N Engl J Med. 2000 Sep 14;343(11):813; author reply 813-4. (PMID: 10991706)
      Clin Chest Med. 1989 Mar;10(1):83-93. (PMID: 2650966)
    • Grant Information:
      31301116 National Natural Science Foundation of China; 11ZA297 Education Department of Sichuan Province; 2019YFS0235 Project of Sichuan Science and Technology
    • Contributed Indexing:
      Keywords: Acute respiratory distress syndrome; Lung injury;Piezo1;apoptosis;Ca2+
    • Accession Number:
      0 (Membrane Proteins)
      0 (Piezo1 protein, rat)
    • Publication Date:
      Date Created: 20190613 Date Completed: 20191226 Latest Revision: 20231012
    • Publication Date:
      20240104
    • Accession Number:
      PMC6558715
    • Accession Number:
      10.1186/s12931-019-1083-1
    • Accession Number:
      31186017