A maize polygalacturonase functions as a suppressor of programmed cell death in plants.

Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol

Original Publication: London : BioMed Central, [2001-

Apoptosis/*physiology ; Plant Proteins/*physiology ; Polygalacturonase/*physiology ; Zea mays/*physiology; Apoptosis/genetics ; Chromosome Mapping ; Chromosomes, Plant ; Genes, Plant ; Leucine ; Plant Proteins/chemistry ; Plant Proteins/genetics ; Polygalacturonase/chemistry ; Polygalacturonase/genetics ; Recombination, Genetic ; Repetitive Sequences, Amino Acid ; Nicotiana/genetics ; Zea mays/enzymology ; Zea mays/genetics ; Zea mays/immunology

Background: The hypersensitive defense response (HR) in plants is a fast, localized necrotic response around the point of pathogen ingress. HR is usually triggered by a pathogen recognition event mediated by a nucleotide-binding site, leucine-rich repeat (NLR) protein. The autoactive maize NLR gene Rp1-D21 confers a spontaneous HR response in the absence of pathogen recognition. Previous work identified a set of loci associated with variation in the strength of Rp1-D21-induced HR. A polygalacturonase gene homolog, here termed ZmPGH1, was identified as a possible causal gene at one of these loci on chromosome 7.
Results: Expression of ZmPGH1 inhibited the HR-inducing activity of both Rp1-D21 and that of another autoactive NLR, RPM1(D505V), in a Nicotiana benthamiana transient expression assay system. Overexpression of ZmPGH1 in a transposon insertion line of maize was associated with suppression of chemically-induced programmed cell death and with suppression of HR induced by Rp1-D21 in maize plants grown in the field.
Conclusions: ZmPGH1 functions as a suppressor of programmed cell death induced by at least two autoactive NLR proteins and by two chemical inducers. These findings deepen our understanding of the control of the HR in plants.

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#0822495 National Science Foundation; #1444503. National Science Foundation

Keywords: Hypersensitive response; Maize; Polygalacturonase

0 (Plant Proteins)
EC 3.2.1.15 (Polygalacturonase)
GMW67QNF9C (Leucine)

Date Created: 20190717 Date Completed: 20190808 Latest Revision: 20231213

20240105

PMC6628502

10.1186/s12870-019-1897-5

31307401