Menu
×
West Ashley Library
9 a.m. - 4 p.m.
Phone: (843) 766-6635
Main Library
9 a.m. - 6 p.m.
Phone: (843) 805-6930
Folly Beach Library
Closed for renovations
Phone: (843) 588-2001
John L. Dart Library
9 a.m. - 6 p.m.
Phone: (843) 722-7550
St. Paul's/Hollywood Library
9 a.m. - 5 p.m.
Phone: (843) 889-3300
Mt. Pleasant Library
9 a.m. – 6 p.m.
Phone: (843) 849-6161
Dorchester Road Library
9 a.m. - 6 p.m.
Phone: (843) 552-6466
Edgar Allan Poe/Sullivan's Island Library
9 a.m. - 6 p.m.
Phone: (843) 883-3914
John's Island Library
9 a.m. - 6 p.m.
Phone: (843) 559-1945
McClellanville Library
Closed for renovations
Phone: (843) 887-3699
Edisto Library
9 a.m. - 3 p.m.
Phone: (843) 869-2355
Wando Mount Pleasant Library
9 a.m. - 6 p.m.
Phone: (843) 805-6888
Otranto Road Library
9 a.m. - 6 p.m.
Phone: (843) 572-4094
Hurd/St. Andrews Library
9 a.m. - 6 p.m.
Phone: (843) 766-2546
Baxter-Patrick James Island
9 a.m. - 6 p.m.
Phone: (843) 795-6679
Bees Ferry West Ashley Library
9 a.m. - 6 p.m.
Phone: (843) 805-6892
Village Library
9 a.m. - 6 p.m.
Phone: (843) 884-9741
Keith Summey North Charleston Library
9 a.m. – 6 p.m.
Phone: (843) 744-2489
Mobile Library
9 a.m. - 5 p.m.
Phone: (843) 805-6909
Today's Hours
West Ashley Library
9 a.m. - 4 p.m.
Phone: (843) 766-6635
Main Library
9 a.m. - 6 p.m.
Phone: (843) 805-6930
Folly Beach Library
Closed for renovations
Phone: (843) 588-2001
John L. Dart Library
9 a.m. - 6 p.m.
Phone: (843) 722-7550
St. Paul's/Hollywood Library
9 a.m. - 5 p.m.
Phone: (843) 889-3300
Mt. Pleasant Library
9 a.m. – 6 p.m.
Phone: (843) 849-6161
Dorchester Road Library
9 a.m. - 6 p.m.
Phone: (843) 552-6466
Edgar Allan Poe/Sullivan's Island Library
9 a.m. - 6 p.m.
Phone: (843) 883-3914
John's Island Library
9 a.m. - 6 p.m.
Phone: (843) 559-1945
McClellanville Library
Closed for renovations
Phone: (843) 887-3699
Edisto Library
9 a.m. - 3 p.m.
Phone: (843) 869-2355
Wando Mount Pleasant Library
9 a.m. - 6 p.m.
Phone: (843) 805-6888
Otranto Road Library
9 a.m. - 6 p.m.
Phone: (843) 572-4094
Hurd/St. Andrews Library
9 a.m. - 6 p.m.
Phone: (843) 766-2546
Baxter-Patrick James Island
9 a.m. - 6 p.m.
Phone: (843) 795-6679
Bees Ferry West Ashley Library
9 a.m. - 6 p.m.
Phone: (843) 805-6892
Village Library
9 a.m. - 6 p.m.
Phone: (843) 884-9741
Keith Summey North Charleston Library
9 a.m. – 6 p.m.
Phone: (843) 744-2489
Mobile Library
9 a.m. - 5 p.m.
Phone: (843) 805-6909
Patron Login
menu
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
Risk prediction for severe disease and better diagnostic accuracy in early dengue infection; the Colombo dengue study.
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- Author(s): Sigera PC;Sigera PC; Amarasekara R; Amarasekara R; Rodrigo C; Rodrigo C; Rajapakse S; Rajapakse S; Weeratunga P; Weeratunga P; De Silva NL; De Silva NL; Huang CH; Huang CH; Sahoo MK; Sahoo MK; Pinsky BA; Pinsky BA; Pinsky BA; Pillai DR; Pillai DR; Tissera HA; Tissera HA; Jayasinghe S; Jayasinghe S; Handunnetti S; Handunnetti S; Fernando SD; Fernando SD
- Source:
BMC infectious diseases [BMC Infect Dis] 2019 Aug 01; Vol. 19 (1), pp. 680. Date of Electronic Publication: 2019 Aug 01.- Publication Type:
Journal Article; Observational Study- Language:
English - Source:
- Additional Information
- Source: Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE
- Publication Information: Original Publication: London : BioMed Central, [2001-
- Subject Terms: Aspartate Aminotransferases/*blood ; Nucleic Acid Amplification Techniques/*methods ; Severe Dengue/*diagnosis ; Severe Dengue/*etiology; Adult ; Bayes Theorem ; Biomarkers/blood ; Cohort Studies ; Dengue/diagnosis ; Dengue Virus/genetics ; Female ; Fever/virology ; Humans ; Immunologic Tests ; Lymphocyte Count ; Male ; Middle Aged ; Prospective Studies ; Real-Time Polymerase Chain Reaction/methods ; Risk Assessment ; Sensitivity and Specificity ; Severe Dengue/blood ; Sri Lanka
- Abstract: Background: A major challenge in dengue management in resource limited settings is the confirmation of diagnosis. Clinical features of dengue often overlap with other infections and molecular diagnostic tools are not readily accessible to clinicians at hospitals. In addition, the prediction of plasma leakage in dengue is also difficult. Hematocrit level and ultrasound scans (combined with clinical parameters) are helpful to detect plasma leakage once it has happened, not before.
Methods: Colombo Dengue Study (CDS) is a prospective cohort study of clinically suspected adult dengue patients recruited from the National hospital of Sri Lanka (within the first 3 days of fever) that aimed to a) identify clinical and basic laboratory test parameters to differentiate dengue from non-dengue fever, b) evaluate the comparative efficacy of loop-mediated isothermal amplification (LAMP) for dengue diagnosis (vs. NS1 antigen test and RT-qPCR) and c) identify early associations that are predictive of plasma leakage or severe dengue. The basic laboratory tests considered here included hematological parameters, serum biochemistry and inflammatory markers.
Results: Only 70% of clinically suspected patients were confirmed as having dengue by either the NS1 antigen test or RT-qPCR. On a Bayesian latent class model which assumes no "gold standard", LAMP performed equally or better than RT-qPCR and NS1 antigen test respectively. When confirmed dengue patients were compared with others, the earlier group had significantly lower lymphocyte counts and higher aspartate aminotransferase levels (AST) within the first 3 days of fever. Confirmed dengue patients with plasma leakage had a lower mean age and a higher median baseline AST level compared to those without plasma leakage (p < 0.05).
Conclusion: Clinical suspicion overestimates the true number of dengue patients. RT-LAMP is a potentially useful low-cost diagnostic tool for dengue diagnosis. Confirmed dengue patients had significantly higher AST levels and lower lymphocyte counts in early disease compared to others. In confirmed dengue patients, younger age and a higher AST level in early infection were associated with subsequent plasma leakage. - References: Nucleic Acids Res. 2000 Jun 15;28(12):E63. (PMID: 10871386)
Biochem Biophys Res Commun. 2001 Nov 23;289(1):150-4. (PMID: 11708792)
Nat Protoc. 2008;3(5):877-82. (PMID: 18451795)
J Clin Microbiol. 2008 Nov;46(11):3653-9. (PMID: 18799705)
Am J Trop Med Hyg. 2010 Sep;83(3):690-5. (PMID: 20810840)
Am J Trop Med Hyg. 2011 Oct;85(4):594-6. (PMID: 21976556)
PLoS One. 2012;7(7):e40633. (PMID: 22844405)
Infect Drug Resist. 2012;5:103-12. (PMID: 22870039)
PLoS Negl Trop Dis. 2012;6(8):e1760. (PMID: 22880140)
PLoS Negl Trop Dis. 2013 Apr 18;7(4):e2116. (PMID: 23638191)
BMC Infect Dis. 2013 Aug 21;13:387. (PMID: 23964963)
Med J Armed Forces India. 2013 Jul;69(3):246-53. (PMID: 24600118)
PLoS Negl Trop Dis. 2014 Jul 10;8(7):e2972. (PMID: 25010515)
BMC Med. 2014 Sep 04;12:147. (PMID: 25259615)
PLoS One. 2015 Sep 18;10(9):e0138694. (PMID: 26384248)
Emerg Infect Dis. 2016 Jul;22(7):1295-7. (PMID: 27184629)
Open Forum Infect Dis. 2017 May 05;4(2):ofx056. (PMID: 28491893)
Front Cell Infect Microbiol. 2017 Jul 12;7:317. (PMID: 28748176)
J Med Virol. 2018 May;90(5):803-809. (PMID: 29315684) - Grant Information: AP /3/2/2017/CG /25 University of Colombo; - University of Calgary,Canada
- Contributed Indexing: Keywords: Dengue; Dengue diagnostics; Dengue virus; LAMP; NS1; RT-qPCR; Severe dengue
- Accession Number: 0 (Biomarkers)
EC 2.6.1.1 (Aspartate Aminotransferases) - Publication Date: Date Created: 20190803 Date Completed: 20191007 Latest Revision: 20200225
- Publication Date: 20240105
- Accession Number: PMC6676631
- Accession Number: 10.1186/s12879-019-4304-9
- Accession Number: 31370795
- Source:
Contact CCPL
Copyright 2022 Charleston County Public Library Powered By EBSCO Stacks 3.3.0 [350.3] | Staff Login
No Comments.