High prevalence of non-O157 Shiga toxin-producing Escherichia coli in beef cattle detected by combining four selective agars.

Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Cattle Diseases/*diagnosis ; Cattle Diseases/*epidemiology ; Escherichia coli Infections/*veterinary ; Shiga-Toxigenic Escherichia coli/*isolation & purification; Animals ; Bacterial Adhesion/genetics ; Cattle ; China/epidemiology ; Culture Media ; Escherichia coli Infections/diagnosis ; Escherichia coli Infections/epidemiology ; Escherichia coli Proteins/genetics ; Farms ; Feces/microbiology ; Genome, Bacterial/genetics ; Prevalence ; Serogroup ; Shiga-Toxigenic Escherichia coli/genetics ; Virulence/genetics

Background: Shiga toxin-producing Escherichia coli (STEC) are emerging foodborne pathogens that are public health concern. Cattle have been identified as the major STEC reservoir. In the present study, we investigated the prevalence and characteristics of STEC strains in beef cattle from a commercial farm in Sichuan province, China.
Results: Among 120 beef cattle fecal samples, stx genes were positive in 90% of samples, as assessed using TaqMan real-time PCR, and 87 (72.5%) samples were confirmed to yield at least one STEC isolate by culture using four selective agars, MacConkey, CHROMagar™ ECC, modified Rainbow® Agar O157, and CHROMagar™ STEC, from which 31, 32, 91, and 73 STEC strains were recovered, respectively. A total of 126 STEC isolates were selected and further characterized. Seventeen different O:H serotypes were identified, all of which belonged to the non-O157 serotypes. One stx 1 subtype (stx 1a ) and three stx 2 subtypes (stx 2a , stx 2c , and stx 2d ) were present among these isolates. The intimin encoding gene eae, and other adherence-associated genes (iha, saa, and paa) were present in 37, 125, 74, and 30 STEC isolates, respectively. Twenty-three isolates carried the virulence gene subA, and only one harbored both cnf1 and cnf2 genes. Three plasmid-origin virulence genes (ehxA, espP, and katP) were present in 111, 111, and 7 isolates, respectively. The 126 STEC isolates were divided into 49 pulsed-field gel electrophoresis (PFGE) patterns.
Conclusions: Our study showed that the joint use of the selective MacConkey and modified Rainbow® Agar O157 agars increased the recovery frequency of non-O157 STEC strains in animal feces, which could be applied to other samples and in regular STEC surveillance. Moreover, the results revealed high genetic diversity of non-O157 STEC strains in beef cattle, some of which might have the potential to cause human diseases.

J Clin Microbiol. 1999 Oct;37(10):3357-61. (PMID: 10488206)
Eur J Clin Microbiol Infect Dis. 2003 Dec;22(12):726-30. (PMID: 14614596)
J Clin Microbiol. 2004 Nov;42(11):4937-46. (PMID: 15528677)
J Food Prot. 2005 Oct;68(10):2224-41. (PMID: 16245735)
J Microbiol Biotechnol. 2010 Jan;20(1):5-14. (PMID: 20134227)
J Clin Microbiol. 2012 Sep;50(9):2951-63. (PMID: 22760050)
J Clin Microbiol. 2013 Feb;51(2):466-71. (PMID: 23175263)
Virulence. 2013 Jul 1;4(5):366-72. (PMID: 23624795)
PLoS One. 2013 Jun 11;8(6):e65537. (PMID: 23776496)
J Vet Sci. 2014;15(3):369-79. (PMID: 23820205)
J Microbiol Methods. 2014 Jan;96:6-11. (PMID: 24211606)
Adv Appl Microbiol. 2014;86:145-97. (PMID: 24377855)
BMC Microbiol. 2014 Jan 06;14:5. (PMID: 24393167)
Epidemiol Infect. 2014 Nov;142(11):2270-80. (PMID: 24398154)
J Clin Microbiol. 2014 Jul;52(7):2346-51. (PMID: 24759708)
Front Cell Infect Microbiol. 2014 Jul 02;4:89. (PMID: 25072032)
J Food Prot. 2014 Aug;77(8):1269-74. (PMID: 25198587)
Appl Environ Microbiol. 2015 Feb;81(4):1397-1405. (PMID: 25527532)
Vet Microbiol. 2015 Feb 25;175(2-4):325-31. (PMID: 25561429)
Int J Food Microbiol. 2015 May 4;200:31-8. (PMID: 25676240)
J Clin Microbiol. 2015 Aug;53(8):2427-32. (PMID: 25926488)
PLoS One. 2015 Aug 13;10(8):e0135446. (PMID: 26270482)
Vet Rec Open. 2015 Jan 20;2(1):e000061. (PMID: 26392887)
BMC Microbiol. 2015 Oct 16;15:213. (PMID: 26475706)
Foodborne Pathog Dis. 2016 Mar;13(3):163-70. (PMID: 26836701)
J Food Prot. 2016 Mar;79(3):413-20. (PMID: 26939651)
Front Microbiol. 2016 Mar 22;7:375. (PMID: 27047483)
Int J Food Microbiol. 2016 Aug 2;230:81-8. (PMID: 27153219)
Diagn Microbiol Infect Dis. 2016 Jul;85(3):302-308. (PMID: 27157987)
Toxins (Basel). 2016 May 18;8(5):. (PMID: 27213452)
J Appl Microbiol. 2016 Oct;121(4):1130-43. (PMID: 27426967)
J Clin Pathol. 2017 Jan;70(1):81-84. (PMID: 27698249)
Front Cell Infect Microbiol. 2016 Nov 02;6:143. (PMID: 27853704)
J Food Prot. 2016 Nov;79(11):1868-1874. (PMID: 28221921)
Epidemiol Infect. 2017 Jun;145(8):1557-1566. (PMID: 28260536)
Gut Pathog. 2017 Apr 21;9:22. (PMID: 28439301)
Front Cell Infect Microbiol. 2017 Apr 28;7:147. (PMID: 28503491)
Microb Pathog. 2017 Aug;109:274-279. (PMID: 28578089)
Lett Appl Microbiol. 2017 Dec;65(6):482-488. (PMID: 28960364)
Int J Food Microbiol. 2018 Feb 2;266:295-300. (PMID: 29274486)
Curr Microbiol. 2018 Jun;75(6):752-759. (PMID: 29423730)
Sci Rep. 2018 Mar 9;8(1):4233. (PMID: 29523817)
Trop Anim Health Prod. 2018 Aug;50(6):1327-1341. (PMID: 29564631)
Sci Rep. 2018 Apr 30;8(1):6756. (PMID: 29712985)
Int J Infect Dis. 2018 Nov;76:82-87. (PMID: 30223088)
Sci Total Environ. 2019 Feb 15;651(Pt 1):203-209. (PMID: 30227290)
J Food Prot. 2019 Jan;82(1):7-21. (PMID: 30586326)
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):3093-7. (PMID: 8385356)

Keywords: Beef cattle; Culture medium; Escherichia coli; Non-O157 STEC; O157:H7; Shiga toxin

0 (Culture Media)
0 (Escherichia coli Proteins)

Date Created: 20190907 Date Completed: 20200706 Latest Revision: 20200706

20240105

PMC6728992

10.1186/s12866-019-1582-8

31488047