Dendropanax morbifera Branch Water Extract Increases the Immunostimulatory Activity of RAW264.7 Macrophages and Primary Mouse Splenocytes.

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  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9812512 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7600 (Electronic) Linking ISSN: 1096620X NLM ISO Abbreviation: J Med Food Subsets: MEDLINE
    • Publication Information:
      Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1998-
    • Subject Terms:
      Adjuvants, Immunologic/*pharmacology ; Araliaceae/*chemistry ; Macrophages/*immunology ; Plant Extracts/*pharmacology ; Polyacetylene Polymer/*pharmacology ; Spleen/*cytology; Animals ; Cytokines/metabolism ; Immunity, Innate ; Macrophages/drug effects ; Male ; Mice ; Mice, Inbred BALB C ; NF-kappa B/metabolism ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/metabolism ; Plant Bark/chemistry ; Plant Leaves/chemistry ; RAW 264.7 Cells ; Signal Transduction ; Spleen/drug effects ; Spleen/immunology
    • Abstract:
      Polyacetylenes in the bark of Dendropanax morbifera trees have been reported to promote immune cell proliferation and to strengthen the innate immune system. The immunomodulatory potential of D. morbifera branch water extract (DBW) was evaluated by determining its effect on cell viability and the expression of cytokines and immune effector molecules in mouse RAW264.7 macrophages and splenocytes. Production of nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cytokines (interleukin [IL]-1 β , IL-2, and IFN- γ ) in RAW264.7 macrophages increased after treatment with DBW. The activation of components of the NF- κ B signaling pathway, including the phospho-I κ B α and the expression and translocation of p65, a subunit of NF- κ B, were also increased in RAW264.7 mouse macrophage cells after treatment with DBW. In addition, when mice were orally administered DBW, splenocyte cytokines and NO production were increased in a dose-dependent manner relative to control-treated mice. Furthermore, natural killer cell activity in DBW-treated mice was determined by lactate dehydrogenase (LDH) release assay. LDH release also increased in response to DBW treatment. Taken together, these results indicate that D. morbifera extract enhances innate immunity by promoting NF- κ B signaling, leading to increased expression of proinflammatory cytokines and effector molecules. DBW therefore has potential therapeutic use in the context of immune stimulation.
    • Contributed Indexing:
      Keywords: Dendropanax morbifera; NK activity; RAW264.7 cells; cytokines; immune stimulation
    • Accession Number:
      0 (Adjuvants, Immunologic)
      0 (Cytokines)
      0 (NF-kappa B)
      0 (Plant Extracts)
      0 (Polyacetylene Polymer)
      31C4KY9ESH (Nitric Oxide)
      EC 1.14.13.39 (Nitric Oxide Synthase Type II)
      EC 1.14.13.39 (Nos2 protein, mouse)
    • Publication Date:
      Date Created: 20191102 Date Completed: 20200330 Latest Revision: 20200330
    • Publication Date:
      20240104
    • Accession Number:
      10.1089/jmf.2019.4424
    • Accession Number:
      31674887