Effect of dietary cellulose supplementation on gut barrier function and apoptosis in a murine model of endotoxemia.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Dietary Supplements*; Apoptosis/*drug effects ; Cellulose/*pharmacology ; Endotoxemia/*metabolism ; Endotoxemia/*pathology ; Intestinal Mucosa/*drug effects ; Intestinal Mucosa/*pathology; Animals ; Cell Proliferation/drug effects ; Disease Models, Animal ; Endotoxemia/microbiology ; Gastrointestinal Microbiome/drug effects ; Gene Expression Regulation/drug effects ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/microbiology ; Male ; Mice ; Mice, Inbred C57BL ; Permeability/drug effects ; Tight Junction Proteins/metabolism
    • Abstract:
      The gut plays a vital role in critical illness, and alterations in the gut structure and function have been reported in endotoxemia and sepsis models. Previously, we have demonstrated a novel link between the diet-induced alteration of the gut microbiome with cellulose and improved outcomes in sepsis. As compared to mice receiving basal fiber (BF) diet, mice that were fed a non-fermentable high fiber (HF) diet demonstrated significant improvement in survival and decreased organ injury in both cecal-ligation and puncture (CLP) and endotoxin sepsis models. To understand if the benefit conferred by HF diet extends to the gut structure and function, we hypothesized that HF diet would be associated with a reduction in sepsis-induced gut epithelial loss and permeability in mice. We demonstrate that the use of dietary cellulose decreased LPS-mediated intestinal hyperpermeability and protected the gut from apoptosis. Furthermore, we noted a significant increase in epithelial cell proliferation, as evidenced by an increase in the percentage of bromodeoxyuridine-positive cells in HF fed mice as compared to BF fed mice. Thus, the use of HF diet is a simple and effective tool that confers benefit in a murine model of sepsis, and understanding the intricate relationship between the epithelial barrier, gut microbiota, and diet will open-up additional therapeutic avenues for the treatment of gut dysfunction in critical illness.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      J Appl Microbiol. 2006 Apr;100(4):846-53. (PMID: 16553741)
      Arch Surg. 1986 Feb;121(2):196-208. (PMID: 3484944)
      Adv Nutr. 2013 Jan 01;4(1):16-28. (PMID: 23319119)
      Mucosal Immunol. 2010 Mar;3(2):148-58. (PMID: 19940845)
      Crit Care Med. 2017 May;45(5):e516-e523. (PMID: 28252538)
      JAMA. 2016 Feb 23;315(8):801-10. (PMID: 26903338)
      J Clin Invest. 2010 Dec;120(12):4332-41. (PMID: 21099116)
      Am J Physiol. 1993 Feb;264(2 Pt 1):G179-86. (PMID: 8447399)
      Trends Mol Med. 2014 Apr;20(4):214-23. (PMID: 24055446)
      Transplantation. 1995 Nov 15;60(9):949-57. (PMID: 7491699)
      Acta Anaesthesiol Belg. 1983 Sep;34(3):209-21. (PMID: 6316704)
      Shock. 2019 Apr;51(4):526-534. (PMID: 30080745)
      BMC Gastroenterol. 2014 Nov 18;14:189. (PMID: 25407511)
      Lancet Respir Med. 2016 Jan;4(1):59-72. (PMID: 26700442)
      Clin Gastroenterol Hepatol. 2013 Sep;11(9):1075-83. (PMID: 23851019)
      Curr Opin Microbiol. 2013 Apr;16(2):221-7. (PMID: 23597788)
      Am J Physiol Gastrointest Liver Physiol. 2003 Nov;285(5):G861-9. (PMID: 12842830)
      Immunity. 2012 Sep 21;37(3):563-73. (PMID: 22981539)
      JAMA. 2014 Oct 8;312(14):1429-1437. (PMID: 25271544)
      Clin Infect Dis. 2012 Oct;55(7):905-14. (PMID: 22718773)
      Pediatr Crit Care Med. 2017 Mar;18(3_suppl Suppl 1):S46-S49. (PMID: 28248833)
      Exp Biol Med (Maywood). 2017 Jan;242(2):127-139. (PMID: 27633573)
      HSR Proc Intensive Care Cardiovasc Anesth. 2012;4(1):21-9. (PMID: 23440328)
      Crit Care Med. 2003 Jun;31(6):1630-7. (PMID: 12794397)
      Nat Rev Immunol. 2009 Nov;9(11):799-809. (PMID: 19855405)
      Cell. 2016 Nov 17;167(5):1339-1353.e21. (PMID: 27863247)
      Infect Control Hosp Epidemiol. 2012 Jan;33(1):14-9. (PMID: 22173517)
      Surgery. 2007 Apr;141(4):481-9. (PMID: 17383525)
      J Exp Med. 2007 Dec 24;204(13):3067-76. (PMID: 18039951)
      Cell. 2013 Dec 19;155(7):1451-63. (PMID: 24315484)
      Cell Host Microbe. 2014 Mar 12;15(3):374-81. (PMID: 24629343)
      Shock. 2016 Jul;46(1):52-9. (PMID: 27299587)
      Shock. 2007 Oct;28(4):384-93. (PMID: 17577136)
      J Pathol. 2009 Jun;218(2):210-21. (PMID: 19235836)
      J Crit Care. 2017 Apr;38:84-91. (PMID: 27866110)
      JAMA. 2002 Apr 3;287(13):1716-21. (PMID: 11926897)
    • Grant Information:
      K08 DK101753 United States DK NIDDK NIH HHS; R03 DK114464 United States DK NIDDK NIH HHS; R01 GM098474 United States GM NIGMS NIH HHS; T32 HD040686 United States HD NICHD NIH HHS; R01 DK120986 United States DK NIDDK NIH HHS
    • Accession Number:
      0 (Tight Junction Proteins)
      9004-34-6 (Cellulose)
    • Publication Date:
      Date Created: 20191203 Date Completed: 20200323 Latest Revision: 20221101
    • Publication Date:
      20240104
    • Accession Number:
      PMC6886840
    • Accession Number:
      10.1371/journal.pone.0224838
    • Accession Number:
      31790417