N-3-oxo-octanoyl-homoserine lactone-mediated priming of resistance to Pseudomonas syringae requires the salicylic acid signaling pathway in Arabidopsis thaliana.

Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Arabidopsis*/genetics ; Arabidopsis*/microbiology; 4-Butyrolactone/*analogs & derivatives ; Plant Immunity/*drug effects ; Pseudomonas syringae/*pathogenicity ; Salicylic Acid/*metabolism; 4-Butyrolactone/pharmacology ; Arabidopsis Proteins/drug effects ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Calmodulin-Binding Proteins/drug effects ; Calmodulin-Binding Proteins/genetics ; Calmodulin-Binding Proteins/metabolism ; Genes, Plant ; Intramolecular Transferases/drug effects ; Intramolecular Transferases/genetics ; Intramolecular Transferases/metabolism ; Plant Diseases/microbiology ; Plant Immunity/genetics ; Quorum Sensing/physiology ; Signal Transduction/drug effects

Backgroud: Many Gram-negative bacteria use N-acyl-homoserine lactones (AHLs) to communicate each other and to coordinate their collective behaviors. Recently, accumulating evidence shows that host plants are able to sense and respond to bacterial AHLs. Once primed, plants are in an altered state that enables plant cells to more quickly and/or strongly respond to subsequent pathogen infection or abiotic stress.
Results: In this study, we report that pretreatment with N-3-oxo-octanoyl-homoserine lactone (3OC8-HSL) confers resistance against the pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (PstDC3000) in Arabidopsis. Pretreatment with 3OC8-HSL and subsequent pathogen invasion triggered an augmented burst of hydrogen peroxide, salicylic acid accumulation, and fortified expression of the pathogenesis-related genes PR1 and PR5. Upon PstDC3000 challenge, plants treated with 3OC8-HSL showed increased activities of defense-related enzymes including peroxidase, catalase, phenylalanine ammonialyase, and superoxide dismutase. In addition, the 3OC8-HSL-primed resistance to PstDC3000 in wild-type plants was impaired in plants expressing the bacterial NahG gene and in the npr1 mutant. Moreover, the expression levels of isochorismate synthases (ICS1), a critical salicylic acid biosynthesis enzyme, and two regulators of its expression, SARD1 and CBP60g, were potentiated by 3OC8-HSL pretreatment followed by pathogen inoculation.
Conclusions: Our data indicate that 3OC8-HSL primes the Arabidopsis defense response upon hemibiotrophic bacterial infection and that 3OC8-HSL-primed resistance is dependent on the SA signaling pathway. These findings may help establish a novel strategy for the control of plant disease.

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2015CB150600 National Basic Research Program of China (973 Program); 31270880 National Science Foundation of China; C2015302020 Natural science Foundation of Hebei

Keywords: Arabidopsis thaliana; N-3-oxo-octanoyl-homoserine lactone (3OC8-HSL); Plant defense; Priming; Quorum sensing (QS); Salicylic acid (SA)

0 (Arabidopsis Proteins)
0 (CBP60g protein, Arabidopsis)
0 (Calmodulin-Binding Proteins)
0 (SARD1 protein, Arabidopsis)
1192-20-7 (homoserine lactone)
147445-32-7 (PR-1 protein, Arabidopsis)
EC 5.4.- (Intramolecular Transferases)
EC 5.4.4.2 (isochorismate synthase)
O414PZ4LPZ (Salicylic Acid)
OL659KIY4X (4-Butyrolactone)

Date Created: 20200130 Date Completed: 20200605 Latest Revision: 20220420

20240105

PMC6986161

10.1186/s12870-019-2228-6

31992205