Menu
×
West Ashley Library
9 a.m. – 7 p.m.
Phone: (843) 766-6635
Main Library
9 a.m. - 8 p.m.
Phone: (843) 805-6930
Folly Beach Library
Closed for renovations
Phone: (843) 588-2001
John L. Dart Library
9 a.m. – 7 p.m.
Phone: (843) 722-7550
St. Paul's/Hollywood Library
9 a.m. - 8 p.m.
Phone: (843) 889-3300
Mt. Pleasant Library
9 a.m. – 8 p.m.
Phone: (843) 849-6161
Dorchester Road Library
9 a.m. - 8 p.m.
Phone: (843) 552-6466
Edgar Allan Poe/Sullivan's Island Library
9 a.m. - 6 p.m.
Phone: (843) 883-3914
John's Island Library
9 a.m. – 8 p.m.
Phone: (843) 559-1945
McClellanville Library
Closed for renovations
Phone: (843) 887-3699
Edisto Library
9 a.m. - 6 p.m.
Phone: (843) 869-2355
Wando Mount Pleasant Library
9 a.m. - 8 p.m.
Phone: (843) 805-6888
Otranto Road Library
9 a.m. - 8 p.m.
Phone: (843) 572-4094
Hurd/St. Andrews Library
9 a.m. - 8 p.m.
Phone: (843) 766-2546
Baxter-Patrick James Island
9 p.m. - 8 p.m.
Phone: (843) 795-6679
Bees Ferry West Ashley Library
9 a.m. - 8 p.m.
Phone: (843) 805-6892
Village Library
9 a.m. - 6 p.m.
Phone: (843) 884-9741
Keith Summey North Charleston Library
9 a.m. – 8 p.m.
Phone: (843) 744-2489
Mobile Library
9 a.m. - 5 p.m.
Phone: (843) 805-6909
Today's Hours
West Ashley Library
9 a.m. – 7 p.m.
Phone: (843) 766-6635
Main Library
9 a.m. - 8 p.m.
Phone: (843) 805-6930
Folly Beach Library
Closed for renovations
Phone: (843) 588-2001
John L. Dart Library
9 a.m. – 7 p.m.
Phone: (843) 722-7550
St. Paul's/Hollywood Library
9 a.m. - 8 p.m.
Phone: (843) 889-3300
Mt. Pleasant Library
9 a.m. – 8 p.m.
Phone: (843) 849-6161
Dorchester Road Library
9 a.m. - 8 p.m.
Phone: (843) 552-6466
Edgar Allan Poe/Sullivan's Island Library
9 a.m. - 6 p.m.
Phone: (843) 883-3914
John's Island Library
9 a.m. – 8 p.m.
Phone: (843) 559-1945
McClellanville Library
Closed for renovations
Phone: (843) 887-3699
Edisto Library
9 a.m. - 6 p.m.
Phone: (843) 869-2355
Wando Mount Pleasant Library
9 a.m. - 8 p.m.
Phone: (843) 805-6888
Otranto Road Library
9 a.m. - 8 p.m.
Phone: (843) 572-4094
Hurd/St. Andrews Library
9 a.m. - 8 p.m.
Phone: (843) 766-2546
Baxter-Patrick James Island
9 p.m. - 8 p.m.
Phone: (843) 795-6679
Bees Ferry West Ashley Library
9 a.m. - 8 p.m.
Phone: (843) 805-6892
Village Library
9 a.m. - 6 p.m.
Phone: (843) 884-9741
Keith Summey North Charleston Library
9 a.m. – 8 p.m.
Phone: (843) 744-2489
Mobile Library
9 a.m. - 5 p.m.
Phone: (843) 805-6909
Patron Login
menu
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
MPV17 does not control cancer cell proliferation.
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- Author(s): Canonne M;Canonne M; Wanet A; Wanet A; Nguyen TTA; Nguyen TTA; Khelfi A; Khelfi A; Ayama-Canden S; Ayama-Canden S; Van Steenbrugge M; Van Steenbrugge M; Fattaccioli A; Fattaccioli A; Sokal E; Sokal E; Najimi M; Najimi M; Arnould T; Arnould T; Renard P; Renard P
- Source:
PloS one [PLoS One] 2020 Mar 10; Vol. 15 (3), pp. e0229834. Date of Electronic Publication: 2020 Mar 10 (Print Publication: 2020).- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
- Publication Information: Original Publication: San Francisco, CA : Public Library of Science
- Subject Terms: Membrane Proteins/*physiology ; Mitochondria/*metabolism ; Mitochondrial Membranes/*metabolism ; Mitochondrial Proteins/*physiology ; Neoplasms/*pathology; Cell Proliferation ; DNA, Mitochondrial/metabolism ; HEK293 Cells ; Hep G2 Cells ; Humans ; Membrane Proteins/genetics ; Mitochondrial Proteins/genetics
- Abstract: MPV17 is described as a mitochondrial inner membrane channel. Although its function remains elusive, mutations in the MPV17 gene result in hepato-cerebral mitochondrial DNA depletion syndrome in humans. In this study, we show that MPV17 silencing does not induce depletion in mitochondrial DNA content in cancer cells. We also show that MPV17 does not control cancer cell proliferation despite the fact that we initially observed a reduced proliferation rate in five MPV17-silenced cancer cell lines with two different shRNAs. However, shRNA-mediated MPV17 knockdown performed in this work provided misguiding results regarding the resulting proliferation phenotype and only a rescue experiment was able to shed definitive light on the implication of MPV17 in cancer cell proliferation. Our results therefore emphasize the caution that is required when scientific conclusions are drawn from a work based on lentiviral vector-based gene silencing and clearly demonstrate the need to systematically perform a rescue experiment in order to ascertain the specific nature of the experimental results.
Competing Interests: The authors have declared that no competing interests exist. - References: J Biol Eng. 2014 Feb 01;8(1):5. (PMID: 24485151)
Biol Open. 2013 Jun 03;2(7):703-10. (PMID: 23862018)
Stem Cells. 2017 Oct;35(10):2184-2197. (PMID: 28795454)
Hum Mutat. 2018 Apr;39(4):461-470. (PMID: 29282788)
Mol Cell. 2003 Mar;11(3):619-33. (PMID: 12667446)
Mol Cancer Ther. 2015 Apr;14(4):877-88. (PMID: 25612620)
Sci Rep. 2018 Jan 8;8(1):93. (PMID: 29311693)
Dis Model Mech. 2019 Mar 14;12(3):. (PMID: 30833296)
Mol Cell Biol. 2012 Dec;32(24):5009-21. (PMID: 23045398)
J Biol Chem. 2008 Jul 11;283(28):19229-34. (PMID: 18480057)
Biol Chem. 2015 Jan;396(1):13-25. (PMID: 25205723)
Science. 2016 Feb 12;351(6274):728-733. (PMID: 26912861)
J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713)
Nat Genet. 2006 May;38(5):570-5. (PMID: 16582910)
J Biol Chem. 2018 Dec 28;293(52):20285-20294. (PMID: 30385507)
J Biol Chem. 2015 May 29;290(22):13840-61. (PMID: 25861990)
Hum Mol Genet. 2010 Mar 15;19(6):1098-107. (PMID: 20042463)
Nucleic Acids Res. 2017 Dec 15;45(22):12808-12815. (PMID: 29106596)
BMC Neurol. 2015 Oct 05;15:179. (PMID: 26437932)
Mol Ther. 2014 Jan;22(1):10-7. (PMID: 24247928)
PLoS Genet. 2016 Jan 13;12(1):e1005779. (PMID: 26760297)
Hum Mol Genet. 1993 Nov;2(11):1829-34. (PMID: 8281143)
Trends Endocrinol Metab. 2017 Nov;28(11):794-806. (PMID: 28797581)
Eukaryot Cell. 2004 Jun;3(3):620-31. (PMID: 15189984) - Accession Number: 0 (DNA, Mitochondrial)
0 (MPV17 protein, human)
0 (Membrane Proteins)
0 (Mitochondrial Proteins) - Publication Date: Date Created: 20200311 Date Completed: 20200720 Latest Revision: 20200720
- Publication Date: 20240105
- Accession Number: PMC7064194
- Accession Number: 10.1371/journal.pone.0229834
- Accession Number: 32155188
- Source:
Contact CCPL
Copyright 2022 Charleston County Public Library Powered By EBSCO Stacks 3.3.0 [350.3] | Staff Login
No Comments.