Suppressor of Fused Inhibits Skin Wound Healing.

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  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101590593 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2162-1918 (Print) Linking ISSN: 21621918 NLM ISO Abbreviation: Adv Wound Care (New Rochelle)
    • Publication Information:
      Original Publication: New Rochelle, N.Y. : Mary Ann Liebert, Inc.
    • Subject Terms:
      Wnt Signaling Pathway* ; Wound Healing*; Fibroblasts/*metabolism ; Keratinocytes/*metabolism ; Repressor Proteins/*metabolism; Animals ; Cell Differentiation ; Disease Models, Animal ; Epidermal Cells ; Epidermis/growth & development ; Epidermis/metabolism ; Fibroblasts/cytology ; Hedgehog Proteins/metabolism ; Keratinocytes/cytology ; Male ; Mice ; Mice, Transgenic ; Skin Aging/pathology ; Skin Aging/physiology ; Transforming Growth Factor beta/metabolism
    • Abstract:
      Objectives: To investigate the effect of suppressor of fused (Sufu) on epidermal and dermal cellular properties and in wound healing. Approach: Transgenic (TG) mice overexpressing human Sufu (hSufu) in the epidermis were applied to investigate the effects of Sufu on epidermal and dermal cellular properties and in wound healing. Results: Histological staining revealed a reduction of epidermal and dermal thickness and an increase of hypodermal adipose tissue in homozygous K14-hSufu TG mice when compared with wild-type (WT) controls. TG mice exhibited significantly delayed skin wound healing. Moreover, the migratory and proliferative capabilities of cultured keratinocytes were decreased in K14-hSufuTG mice. Transforming growth factor-β treatment increased the expression of α-smooth muscle actin more in WT than in TG fibroblasts. Sufu overexpression significantly decreased the expression of β-catenin, glioma transcription factor 1 (Gli1), and matrix metalloproteinase-3 in wounds of K14-hSufu TG mice when compared with controls, probably indicating a delaying effect of Sufu on wound healing via blocking the hedgehog (Hh)/Gli and Wnt/β-catenin pathway. Innovation: Our results indicate a new property of Sufu in the process of skin wound healing. It provides an important basis for Sufu as a potential target for skin wound healing. Conclusion: Our findings suggest that Sufu overexpression in the epidermis impairs wound healing via dampening the Hh/Gli and Wnt/β-catenin signaling pathway. These data provide an important basis for further analyses of Sufu in skin wound healing.
      Competing Interests: The article was written by the authors and ghostwriting services were not used.
      (Copyright 2020, Mary Ann Liebert, Inc., publishers.)
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    • Contributed Indexing:
      Keywords: skin; suppressor of fused; transgenic mouse; wound healing; β-catenin
    • Accession Number:
      0 (Hedgehog Proteins)
      0 (Repressor Proteins)
      0 (Sufu protein, mouse)
      0 (Transforming Growth Factor beta)
    • Publication Date:
      Date Created: 20200401 Date Completed: 20201125 Latest Revision: 20201125
    • Publication Date:
      20240105
    • Accession Number:
      PMC7099415
    • Accession Number:
      10.1089/wound.2018.0890
    • Accession Number:
      32226648