Sensory nerve-deficient microenvironment impairs tooth homeostasis by inducing apoptosis of dental pulp stem cells.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Source:
      Publisher: Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications Country of Publication: England NLM ID: 9105195 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2184 (Electronic) Linking ISSN: 09607722 NLM ISO Abbreviation: Cell Prolif Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Oxford, England] : Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications, 1991-
    • Subject Terms:
    • Abstract:
      Objectives: The aim of this study is to investigate the role of sensory nerve in tooth homeostasis and its effect on mesenchymal stromal/stem cells (MSCs) in dental pulp.
      Materials and Methods: We established the rat denervated incisor models to identify the morphological and histological changes of tooth. The groups were as follows: IANx (inferior alveolar nerve section), SCGx (superior cervical ganglion removal), IANx + SCGx and Sham group. The biological behaviour of dental pulp stromal/stem cells (DPSCs) was evaluated. Finally, we applied activin B to DPSCs from sensory nerve-deficient microenvironment to analyse the changes of proliferation and apoptosis.
      Results: Incisor of IANx and IANx + SCGx groups exhibited obvious disorganized tooth structure, while SCGx group only showed slight decrease of dentin thickness, implying sensory nerve, not sympathetic nerve, contributes to the tooth homeostasis. Moreover, we found sensory nerve injury led to disfunction of DPSCs via activin B/SMAD2/3 signalling in vitro. Supplementing activin B promoted proliferation and reduced apoptosis of DPSCs in sensory nerve-deficient microenvironment.
      Conclusions: This research first demonstrates that sensory nerve-deficient microenvironment impairs tooth haemostasis by inducing apoptosis of DPSCs via activin B/SMAD2/3 signalling. Our study provides the evidence for the crucial role of sensory nerve in tooth homeostasis.
      (© 2020 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.)
    • References:
      J Clin Periodontol. 2016 Jan;43(1):72-84. (PMID: 26719165)
      J Dent Res. 1969 Sep-Oct;48(5):969. (PMID: 5261318)
      Cold Spring Harb Perspect Biol. 2018 Jan 2;10(1):. (PMID: 28246184)
      Int J Mol Sci. 2018 Mar 28;19(4):. (PMID: 29597317)
      Cell Rep. 2019 Sep 10;28(11):2757-2766.e5. (PMID: 31509739)
      ACS Biomater Sci Eng. 2017 Apr 10;3(4):590-600. (PMID: 33429626)
      Dev Dyn. 2005 Nov;234(3):482-8. (PMID: 16217735)
      Glia. 2019 Nov;67(11):2203-2215. (PMID: 31215712)
      J Dent Res. 2018 Jul;97(8):954-961. (PMID: 29513607)
      Front Cell Dev Biol. 2017 Dec 05;5:103. (PMID: 29259970)
      Nature. 2014 Sep 25;513(7519):551-4. (PMID: 25079316)
      J Dent Res. 1977 Jul;56(7):827-34. (PMID: 269162)
      Biochim Biophys Acta Gen Subj. 2017 Jan;1861(1 Pt A):3011-3018. (PMID: 27693126)
      Cell Adh Migr. 2016 Nov;10(6):618-626. (PMID: 27715429)
      Cell Stem Cell. 2016 Oct 6;19(4):433-448. (PMID: 27376984)
      PLoS One. 2013 Jun 14;8(6):e66863. (PMID: 23799134)
      Stem Cell Res Ther. 2018 Dec 7;9(1):342. (PMID: 30526677)
      J Neurosci Methods. 2010 Sep 30;192(1):22-33. (PMID: 20637235)
      Genes Dev. 1998 Aug 15;12(16):2636-49. (PMID: 9716414)
      Sci Transl Med. 2018 Aug 22;10(455):. (PMID: 30135248)
      Nature. 2014 Oct 2;514(7520):98-101. (PMID: 25079320)
      Trends Neurosci. 2012 Nov;35(11):691-9. (PMID: 22989534)
      Cell Tissue Res. 1983;230(1):197-203. (PMID: 6850763)
      Cell. 2008 Feb 22;132(4):598-611. (PMID: 18295578)
      Biochem Biophys Res Commun. 2015 Nov 6;467(1):21-6. (PMID: 26427874)
      Nature. 2010 Aug 12;466(7308):829-34. (PMID: 20703299)
      Curr Osteoporos Rep. 2016 Jun;14(3):87-94. (PMID: 27048473)
      Cell Prolif. 2020 May;53(5):e12803. (PMID: 32246537)
      Biomaterials. 2019 Mar;196:18-30. (PMID: 29122279)
      Sci Rep. 2017 Jul 12;7(1):5254. (PMID: 28701802)
      J Endod. 2015 Jul;41(7):1056-60. (PMID: 25702857)
      Cell Stem Cell. 2014 Feb 6;14(2):160-73. (PMID: 24506883)
      Cell Rep. 2018 Jun 5;23(10):3102-3111. (PMID: 29874594)
      Eur Rev Med Pharmacol Sci. 2012 Sep;16(9):1218-21. (PMID: 23047505)
      J Anat. 2010 Jul;217(1):16-25. (PMID: 20456522)
      EMBO J. 2015 Jun 12;34(12):1648-60. (PMID: 25916827)
      Cell. 2011 Nov 23;147(5):1146-58. (PMID: 22118468)
      J Dent Res. 1977 Jul;56(7):835-40. (PMID: 269163)
      Int J Biochem Cell Biol. 2017 Jun;87:69-76. (PMID: 28396222)
      Sci Rep. 2019 Mar 12;9(1):4245. (PMID: 30862799)
      Cell Stem Cell. 2011 May 6;8(5):552-65. (PMID: 21549329)
      Nat Commun. 2017 Jul 27;8:15990. (PMID: 28748922)
      Dev Cell. 2012 Aug 14;23(2):317-28. (PMID: 22819339)
      J Tissue Eng Regen Med. 2017 Jun;11(6):1792-1805. (PMID: 26455905)
    • Grant Information:
      2016YFC1101400 National Key Research and Development Program of China; 2017YFA0104800 National Key Research and Development Program of China; 81991504 National Natural Science Foundation of China; 81870768 National Natural Science Foundation of China; 81570937 National Natural Science Foundation of China; 201803040008 Guangzhou Science, Technology and Innovation Commission
    • Contributed Indexing:
      Keywords: dental pulp stem cells; inferior alveolar nerve; microenvironment; nervous system; tooth
    • Publication Date:
      Date Created: 20200405 Date Completed: 20200615 Latest Revision: 20231111
    • Publication Date:
      20240105
    • Accession Number:
      PMC7260073
    • Accession Number:
      10.1111/cpr.12803
    • Accession Number:
      32246537