Decorin inhibits glucose-induced lens epithelial cell apoptosis via suppressing p22phox-p38 MAPK signaling pathway.

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  • Author(s): Du S;Du S; Shao J; Shao J; Shao J; Xie D; Xie D; Zhang F; Zhang F
  • Source:
    PloS one [PLoS One] 2020 Apr 27; Vol. 15 (4), pp. e0224251. Date of Electronic Publication: 2020 Apr 27 (Print Publication: 2020).
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Apoptosis*; Anterior Capsule of the Lens/*cytology ; Antioxidants/*pharmacology ; Decorin/*pharmacology ; Epithelial Cells/*drug effects ; p38 Mitogen-Activated Protein Kinases/*metabolism; Aged ; Cells, Cultured ; Epithelial Cells/metabolism ; Female ; Glucose/pharmacology ; Humans ; MAP Kinase Signaling System ; Male ; Middle Aged ; Oxidative Stress ; Reactive Oxygen Species/metabolism
    • Abstract:
      Purpose: To determine the effect of decorin on oxidative stress and apoptosis of human lens epithelial (HLE) cells under high glucose condition.
      Methods: HLE cell line (HLEB3) was incubated in normal glucose (5.5 mM) or high glucose (60 mM) medium. Decorin (50 nM) was applied 2 hours before high glucose medium was added. Apoptosis detection was executed by flow cytometry and western blotting (analysis of bcl-2 and bax). Oxidative stress level was measured by the generation of reactive oxygen species (ROS), glutathione peroxidase (GSH) and superoxide dismutase (SOD). P38 mitogen-activated protein kinase (MAPK) phosphorylation, the expression of p22phox of HLE cells and human lens anterior capsules were detected by western blotting. Small interfering RNA transfection to p22phox and p38 MAPK was also carried out on HLEB3.
      Results: High glucose caused HLE cells oxidative stress and apoptosis exhibiting the increase of apoptotic cells and ROS production and decrease of bcl-2/bax ratio, GSH/GSSG ration and SOD activity. P22phox and phospho-p38 MAPK were upregulated in high glucose treated HLEB3 cells. Knocking down p22phox or p38 by siRNAs can reduce high glucose induced cell apoptosis and oxidative stress level. Silencing p22phox by siRNA can downregulate the phosphorylation of p38 MAPK. Decorin can inhibit the apoptosis, oxidative stress level and the induction of p22phox and phospho-p38 of HLEB3 induced by high glucose. Furthermore, the expression of p22phox and p38 were found significantly increased in lens anterior capsules of diabetic cataract patients compared to that of normal age-related cataract patients.
      Conclusions: Results showed that p22phox-p38 pathway may be participated in high glucose induced lens epithelial cell injury, decorin may inhibit the high glucose induced apoptosis and oxidative stress injury by suppressing this pathway in part.
      Competing Interests: The authors have declared that no competing interests exist.
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    • Accession Number:
      0 (Antioxidants)
      0 (Decorin)
      0 (Reactive Oxygen Species)
      EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
      IY9XDZ35W2 (Glucose)
    • Publication Date:
      Date Created: 20200428 Date Completed: 20200629 Latest Revision: 20200629
    • Publication Date:
      20240105
    • Accession Number:
      PMC7185589
    • Accession Number:
      10.1371/journal.pone.0224251
    • Accession Number:
      32339204